Effect of thymoquinone on the resistance of Cronobacter sakazakii to environmental stresses and antibiotics
Effect of thymoquinone on the resistance of Cronobacter sakazakii to environmental stresses and antibiotics
复制标题
百里醌对坂崎克罗诺杆菌对环境胁迫和抗生素耐药性的影响
DOI：
10.1016/j.foodcont.2019.106944
复制
发表时间：
2020-03-01
期刊：
影响因子：
6
通讯作者：
Shi, Chao
中科院分区：
文献类型：
--
作者：
Chen, Yifei;Wen, Qiwu;Shi, Chao
坂崎克罗伊氏菌是一种食源性细菌病原体，对广泛的应激条件具有抗性。本文研究了麝香草醌（TQ）对环境胁迫和抗生素胁迫的影响。salcazaltine。测定TQ对C. sakazakii，在胰蛋白胨大豆肉汤（TSB）和重组婴儿配方奶粉（RIF）中研究了TQ暴露后热、酸、干燥和高渗应激条件下细菌存活百分比的变化。我们还监测了TQ处理的细菌中应激耐受相关基因表达的变化，并使用最小抑制浓度测定来检查C.在TQ处理后，选择TQ为200、400和600 μ M的SIG用于测定。TQ处理C.在TSB和RIF中，与未处理的对照相比，坂崎菌株在暴露于高温（50 ℃、55 ℃和60 ℃）、酸性pH（类似于3.3）、高渗透压（a（w）= 0.81）和干燥（P < 0.05）后的存活率显著降低。TQ处理也增加了C.氨苄西林和头孢西丁。此外，实时定量聚合酶链反应（RT-qPCR）分析显示，与胁迫耐受性相关的15个基因的转录水平下调TQ治疗（600 μ M）后。因此，我们的研究结果表明，TQ有显着的负面影响，C。为TQ作为天然抗菌剂在食品生产中的实际应用提供理论依据。
Cronobacter sakazakii is a foodborne bacterial pathogen with resistance to a wide range of stress conditions. Here, we investigated the effects of thymoquinone (TQ) on the environmental and antibiotic stress tolerance of C. salcazalcii. After determining the subinhibitory concentrations (SICs) of TQ against C. sakazakii, changes in the percent survival of bacteria under heat, acid, desiccation, and hyperosmotic stress following exposure to TQ were investigated in tryptone soya broth (TSB) and reconstituted infant formula (RIF). We also monitored changes in the expression of stress tolerance-associated genes in the TQ-treated bacteria, and used a minimum inhibitory concentration assay to examine changes in the antibiotic sensitivity of C. sakazakii following TQ treatment, SIGs of TQ of 200, 400, and 600 mu M were selected for use in the assays. TQ-treated C. sakazakii strains showed a significant decrease in survival following exposure to high temperatures (50 degrees C, 55 degrees C, and 60 degrees C), acidic pH (similar to 3.3), high osmotic pressure (a(w) = 0.81), and desiccation (P < 0.05) compared with untreated controls in both TSB and RIF. TQ treatment also increased the sensitivity of C. sakazakii to ampicillin and cefoxitin. Moreover, real-time quantitative polymerase chain reaction (RT-qPCR) analysis revealed that the transcription levels of 15 genes associated with stress tolerance was downregulated following TQ treatment (600 mu M). Therefore, our findings suggest that TQ has a significant negative impact on the stress tolerance of C. sakazakii, and provide a theoretical basis for the practical application of TQ as a natural antimicrobial agent in food production.