Immunoglobulin G expression in carcinomas and cancer cell lines
Immunoglobulin G expression in carcinomas and cancer cell lines
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DOI：
10.1096/fj.07-8073com
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发表时间：
2007-09-01
期刊：
影响因子：
4.8
通讯作者：
Gu, Jiang
中科院分区：
文献类型：
--
作者：
Chen, Zhengshan;Gu, Jiang
免疫球蛋白基因仅由分化的B淋巴细胞产生的传统观点已受到挑战，因为已发现免疫球蛋白基因在非造血性人类癌细胞中表达。然而，这一现象尚未被广泛接受，对这一新发现的概念的了解也是有限的。在这项研究中，我们研究了6个细胞系，包括上皮性癌细胞，以及66例增生性疾病、腺瘤和癌组织中的IgG1重链(IGHG1)恒定区基因和蛋白的表达。我们还通过检测RAG1(重组激活基因1)、RAG2和AID(激活诱导型胞苷脱氨酶)的表达，探讨了这些细胞产生免疫球蛋白的机制。用巢式RT-PCR方法检测癌细胞中IGHG1恒定区和I-Gamma-C不育转录本的mRNA，用免疫荧光和Western印迹方法检测Ig-γ和Ig-kappa蛋白。在手术切除的癌组织中，我们通过原位杂交和激光显微切割辅助的巢式RT-PCR检测了IGHG1恒定区的mRNA。免疫组织化学方法检测Ig-γ和Ig-kappa蛋白。在这些癌细胞系中还发现了IgH和IGL基因的V(D)J重组，S伽玛1/2-S穆开关环，以及RAG1RAG2的表达。这些数据表明，癌细胞能够产生免疫球蛋白。由于其潜在的生物学和临床意义，这一现象值得进一步研究。免疫球蛋白G在肿瘤和癌细胞系中的表达。
The traditional view that immunoglobulin is produced only by differentiated B lymphocytes has been challenged as immunoglobulin genes have been found to be expressed in nonhematopoietic human cancer cells. However, this phenomenon has not been widely accepted, and knowledge about this newly discovered concept is limited. In this study, we investigated the IgG1 heavy chain (IGHG1) constant region gene and IgG protein expression in 6 cell lines, including epithelial cancer cells, and in tissues from 66 hyperplasias, adenomas, and carcinomas. We also studied the mechanism of IgG production in these cells by examining the expression of RAG1 ( recombination activating gene 1), RAG2, and AID (activation-induced cytidine deaminase). In cancer cell lines, mRNA of the IGHG1 constant region and I gamma-C gamma sterile transcript were detected by nested RT-PCR, and Ig gamma and Ig kappa proteins were detected by immunofluorescence and Western blot. In surgically resected carcinoma tissues, we detected mRNA of the IGHG1 constant region by in situ hybridization, and by laser microdissection-assisted nested RT-PCR. Ig gamma and Ig kappa proteins were detected by immunohistochemistry. The V( D)J recombination of IgH and IgL loci, the S gamma 1/2-S mu switch circle, and the expression of RAG1 and RAG2 were also found in these cancer cell lines. These data suggest that cancer cells are capable of producing IgG. Because of its potential biological and clinical significance, this phenomenon warrants further investigation.-Chen, Z. and Gu, J. Immunoglobulin G expression in carcinomas and cancer cell lines.