Double‐stranded RNA induces S100 gene expression by a cycloheximide‐sensitive factor
Double‐stranded RNA induces S100 gene expression by a cycloheximide‐sensitive factor
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DOI：
10.1016/j.febslet.2011.12.022
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发表时间：
2012-01
期刊：
影响因子：
3.5
通讯作者：
A. Voss;K. Gescher;A. Hensel;W. Nacken;K. Zänker;C. Kerkhoff
中科院分区：
文献类型：
--
作者：
A. Voss;K. Gescher;A. Hensel;W. Nacken;K. Zänker;C. Kerkhoff
病毒双链RNA（dsRNA）及其合成类似物polyI：C通过多种途径被识别并诱导与炎症相关的基因表达。在本研究中，我们证明了polyI：C诱导的损伤相关分子模式（DAMP）分子S100 A8和S100 A9的基因表达，而其他S100基因不受影响。放线菌酮和布雷菲德菌素A处理揭示了S100 A8和S100 A9作为次级应答基因的表达以及聚I：C诱导的细胞因子的参与。几种I型和III型干扰素如IFNβ、IL-20、IL-24和IFNλ/IL-29响应于polyI：C而表达，然而，它们不能诱导S100 A8和S100 A9基因表达。这些数据表明危险分子S100 A8/A9参与了对病毒的抵抗。
Viral double-stranded RNA (dsRNA) and its synthetic analog polyI:C are recognized via multiple pathways and induce the expression of genes related to inflammation. In the present study, we demonstrated the polyI:C-induced gene expression of the damage associated molecular pattern (DAMP) molecules S100A8 and S100A9, while other S100 genes were not affected. Cycloheximide and Brefeldin A treatment revealed both the expression of S100A8 and S100A9 as secondary response genes and the involvement of polyI:C-induced cytokines herein. Several type I and type III interferons such as IFNβ, IL-20, IL-24, and IFNλ/IL-29 were expressed in response to polyI:C, however, they failed to induce S100A8 and S100A9 gene expression. These data indicate the involvement of the danger molecule S100A8/A9 in the resistance against viruses.