PCR-suppression effect: Kinetic analysis and application to representative or long-molecule biased PCR-based amplification of complex samples
PCR-suppression effect: Kinetic analysis and application to representative or long-molecule biased PCR-based amplification of complex samples
复制标题
PCR 抑制效应：动力学分析及其在复杂样品的代表性或长分子偏倚 PCR 扩增中的应用
DOI：
10.1016/j.jbiotec.2006.10.018
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发表时间：
2007-02-20
影响因子：
4.1
通讯作者：
Yang, Wei-Jun
中科院分区：
文献类型：
--
作者：
Dai, Zhong-Min;Zhu, Xiao-Jing;Yang, Wei-Jun
In the present study, we analyzed the kinetics of the PCR-suppression effect (PS-effect) and firstly demonstrate that lower annealing temperature enhances PS-effect. Furthermore, we controlled the average size of complex PCR products over a wide range, and simultaneously amplified targets from 0.25 to 10 kb by regulating the degree of suppression in a single-primer PCR. In addition, we describe an improved template-switching full-length cDNA synthesis method that greatly reduces truncated cDNA. This study provides a general guide for the design of PS-effect related PCR and is useful for representative or long-transcript enriched cDNA library construction, especially when only a small amount of total RNA is available. (c) 2006 Published by Elsevier B.V.
