Immunostaining for allatotropin and allatostatin-A and -C in the mosquitoes Aedes aegypti and Anopheles albimanus
Immunostaining for allatotropin and allatostatin-A and -C in the mosquitoes Aedes aegypti and Anopheles albimanus
复制标题
DOI：
10.1007/s00441-005-1133-5
复制
发表时间：
2005-07-01
影响因子：
3.6
通讯作者：
Noriega, FG
中科院分区：
文献类型：
--
作者：
Hernández-Martínez, S;Li, YP;Noriega, FG
用激光共聚焦扫描显微镜对埃及伊蚊和白纹伊蚊成虫体内的3种神经肽家族在这两个物种中，这三种多肽的特定免疫染色模式是相似的。抗AT、AS-A和AS-C的抗血清在大脑各原脑叶的细胞内呈强阳性反应，在每个腹侧神经节和分布于胸、腹部各组织的神经元投射中呈阳性反应。仅AS-A抗血清标记中肠内分泌细胞。这些多肽的分布支持了它们在两个物种中发挥多种调节作用的概念。
Confocal laser-scanning microscopy was used to carry out a comparative study of the immunostaining for three families of neuropeptides, viz., allatostatin-A (AS-A), allatostatin-C (AS-C) and allatotropin (AT), in adult female mosquitoes of Aedes aegypti and Anopheles albimanus. The specific patterns of immunostaining for each of the three peptides were similar in both species. The antisera raised against AT, AS-A, and AS-C revealed intense immunoreactivity in the cells of each protocerebral lobe of the brain and stained cells in each of the ventral ganglia and neuronal projections innervating various thoracic and abdominal tissues. Only the AS-A antiserum labeled immunoreactive endocrine cells in the midgut. The distribution of the peptides supports the concept that they play multiple regulatory roles in both species.