Screening of differentially expressed genes and identification of AMACR as a prognostic marker in prostate cancer
Screening of differentially expressed genes and identification of AMACR as a prognostic marker in prostate cancer
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DOI：
10.1111/and.14067
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发表时间：
2021-04-16
期刊：
影响因子：
2.4
通讯作者：
Wu，Jifeng
中科院分区：
文献类型：
--
作者：
Fu，Ping;Bu，Chunying;Wu，Jifeng
前列腺癌是全球男性中第二常见的癌症，预计 2020 年美国将有 191,930 例新发病例和 33,330 例死亡。前列腺癌在男性中非常常见，大约 12.1% 的男性一生中会患上这种癌症，据报道老年男性和非裔美国男性的风险较高。已发现基因失调与癌症的发展密切相关。为了进一步了解基因失调如何影响前列腺癌，我们在研究中应用生物信息学工具分析了来自基因表达综合库 (GEO) 的三个前列腺癌基因分析数据集。首先，我们确定了三个基因分析数据集共有的共同差异表达基因（DEG），构建了蛋白质-蛋白质相互作用网络并确定了前 10 个中心基因。 TCGA和人类蛋白质图谱数据库中进一步的DEGs验证确定AMAC为核心基因。然后我们分析了 AMACR 在前列腺癌细胞系中的作用，发现 AMACR 敲低导致细胞增殖减少和细胞凋亡增加。这些结果表明 AMACR 在前列腺癌中具有致癌作用，并且它可能是诊断前列腺癌的潜在生物标志物。
Prostate cancer, the second most common cancer found in male over the world, was estimated to have 191,930 new cases and 33,330 deaths in 2020 in the United States. Prostate cancer is very common in male, about 12.1% of men will acquire this cancer in their lifetime, and a higher risk was reported in older men and African American men. Gene deregulations have been found to be extensively associated with cancer development. To gain further insight into how gene deregulation affects prostate cancer, we analysed three gene profiling datasets of prostate cancer from Gene Expression Omnibus (GEO) applying bioinformatic tools in our study. Firstly, we identified common differently expressed genes (DEGs) shared by the three gene profiling datasets, constructed protein–protein interaction network and determined top 10 hub genes. Further DEGs validation in TCGA and Human Protein Atlas Database identifiedAMACRas the core gene. We then analysed the role of AMACR in prostate cancer cell lines and found thatAMACR‐knockdown resulted in the decreased cell proliferation and increased apoptosis. These results suggest an oncogenic role of AMACR in prostate cancer, and it could be a potential biomarker for the diagnosis of prostate cancer.