Expression Profiling Reveals Developmentally Regulated lncRNA Repertoire in the Mouse Male Germline
Expression Profiling Reveals Developmentally Regulated lncRNA Repertoire in the Mouse Male Germline
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DOI：
10.1095/biolreprod.113.113308
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发表时间：
2013-11-01
影响因子：
3.6
通讯作者：
Yan, Wei
中科院分区：
文献类型：
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作者：
Bao, Jianqiang;Wu, Jingwen;Yan, Wei
在哺乳动物中，大型非编码rna （lncRNAs）的转录组被认为比信使rna （mrna）的转录组大。一些lncRNAs，特别是大的基因间非编码rna (lincRNAs)，通过结合染色质修饰蛋白复合物和调节蛋白质编码基因的表达参与表观遗传调控。鉴于表观遗传调控在雄性生殖系发育中起着关键作用，我们开始使用微阵列分析在雄性生殖系重编程和出生后发育过程中lncrna和mrna的表达谱。我们发现了数千种lncrna和数百种lncrna在男性生殖细胞发育的六个关键时间点上调或下调。此外，高度调控的lncrna与附近的(
In mammals, the transcriptome of large noncoding RNAs (lncRNAs) is believed to be greater than that of messenger RNAs (mRNAs). Some lncRNAs, especially large intergenic noncoding RNAs (lincRNAs), participate in epigenetic regulation by binding chromatin-modifying protein complexes and regulating protein-coding gene expression. Given that epigenetic regulation plays a critical role in male germline development, we embarked on expression profiling of both lncRNAs and mRNAs during male germline reprogramming and postnatal development using microarray analyses. We identified thousands of lncRNAs and hundreds of lincRNAs that are either up-or downregulated at six critical time points during male germ cell development. In addition, highly regulated lncRNAs were correlated with nearby (