In situ preparation of gold nanoparticle-loaded lysozyme-dextran nanogels and applications for cell imaging and drug delivery
In situ preparation of gold nanoparticle-loaded lysozyme-dextran nanogels and applications for cell imaging and drug delivery
复制标题
金纳米颗粒负载溶菌酶-葡聚糖纳米凝胶的原位制备及其在细胞成像和药物递送中的应用
DOI：
10.1039/c3nr00178d
复制
发表时间：
2013-01-01
期刊：
影响因子：
6.7
通讯作者：
Yao, Ping
中科院分区：
文献类型：
--
作者：
Cai, Huanxin;Yao, Ping
本研究发展了一种有效、绿色、简便的制备载金蛋白多糖纳米凝胶的方法。以溶菌酶-葡聚糖纳米凝胶为还原剂和稳定剂，制备了生物相容的金纳米颗粒负载溶菌酶-葡聚糖（Au@Lys-Dex）纳米凝胶。溶菌酶-葡聚糖纳米凝胶具有约200 nm的尺寸和溶菌酶核和葡聚糖壳的结构。在pH值为4左右时，由于静电和配位作用，AuCl 4-离子被溶菌酶吸附并局部富集。当溶液在紫外线照射下，AuCl 4-离子被还原为金纳米粒子在原位的溶剂化电子和活性自由基产生的芳香族氨基酸残基的溶菌酶。所产生的具有约8 nm尺寸的金纳米颗粒被捕获在纳米凝胶内，并且Au@Lys-Dex纳米凝胶由于葡聚糖壳而可良好分散。抗肿瘤药物阿霉素可以通过扩散有效地负载在Au@Lys-Dex纳米凝胶中。体外研究表明，阿霉素负载Au@Lys-Dex纳米凝胶具有与游离阿霉素相同的抗肿瘤活性。纳米凝胶可用作光学细胞成像中的对比剂，其中同步呈现金纳米颗粒和释放的阿霉素的亚细胞分布的直接视觉图像。双功能载药Au@Lys-Dex纳米凝胶是一种有前途的同时给药和生物医学成像系统。
An effective, green, and facile approach to synthesize gold nanoparticle-loaded protein-polysaccharide nanogels was developed in this study. Biocompatible gold nanoparticle-loaded lysozyme-dextran (Au@Lys-Dex) nanogels were produced using lysozyme-dextran nanogels as reducing and stabilizing agents. Lysozyme-dextran nanogels have a size of about 200 nm and a structure of lysozyme core and dextran shell. At pH around 4, AuCl4- ions are attracted and locally enriched by lysozyme due to the electrostatic and coordination interactions. When the solution is under UV irradiation, the AuCl4- ions are reduced to gold nanoparticles in situ by solvated electrons and reactive radicals produced from aromatic amino acid residues in the lysozyme. The produced gold nanoparticles with a size of about 8 nm are trapped inside the nanogels and the Au@Lys-Dex nanogels are well dispersible by virtue of the dextran shell. Antitumor drug, doxorubicin, can be loaded effectively inside Au@Lys-Dex nanogels via diffusion. In vitro study demonstrates the doxorubicin loaded Au@Lys-Dex nanogels have the same antitumor activity as free doxorubicin. The nanogels can be used as a contrasting agent in optical cell imaging, in which direct visual images of the subcellular distributions of the gold nanoparticles and the released doxorubicin are presented synchronously. The dual functional drug loaded Au@Lys-Dex nanogels are a promising system for simultaneous drug delivery and biomedical imaging.