Obesogenic diet intake during pregnancy programs aberrant synaptic plasticity and addiction-like behavior to a palatable food in offspring
Obesogenic diet intake during pregnancy programs aberrant synaptic plasticity and addiction-like behavior to a palatable food in offspring
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DOI：
10.1016/j.bbr.2017.05.014
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发表时间：
2017-07-14
影响因子：
2.7
通讯作者：
Garza-Ocanas, Lourdes
中科院分区：
文献类型：
--
作者：
Camacho, Alberto;Montalvo-Martinez, Larisa;Garza-Ocanas, Lourdes
背景食物条件行为需要奖赏系统中的谷氨酸能神经传递的可塑性，涉及包括α-氨基-3-羟基-5-甲基异恶唑4-丙酸酯受体（AMPA）、N-甲基-天冬氨酸（NMDA）和代谢型谷氨酸2，3（mGlur 2，3）的表达的变化。然而，在发育过程中的能量密集可口的食物偏好的突触标记物的变化的作用还没有被描述。在这里，我们确定的营养计划在妊娠期脂肪食物的选择使用条件性位置偏好（CPP）测试和操作性训练反应和其对nucleus nervebens（Nac）壳和前额叶皮层（PFC）中的神经元标记物的影响。我们的数据表明，大鼠表现出对可口的脂肪食物的偏好，并且与普通饲料相比，热量摄入增加。值得注意的是，74%的大鼠表现出对脂肪食物摄入的偏好与积极的HFD配对评分相关，而26%的大鼠未能获得HFD条件。此外，在操作性训练反应时间表（FR 1，FR 5和PR）下训练的雄性大鼠显示出高和低反应组为食物而工作。值得注意的是，与普通饲料喂养的后代相比，雌性大鼠的高热量营养方案导致雌性后代的肥胖加重。最后，我们发现，一个操作性的训练反应可口的饼干与上调mGlur 2，3在NAC壳和PFC的雄性大鼠和雌性后代。此外，我们发现选择性Nr 1上调NAC壳和PFC的女性后代。我们的数据表明，通过高热量摄入的营养规划导致了为食物而工作的激励动机和中脑边缘系统中突触可塑性的改变。
Contextual food conditioned behaviors require plasticity of glutamatergic neurotransmission in the reward system, involving changes in the expression of including a-amino-3-hydroxy-5-methylisoxazole 4-propionate receptors (AMPA), N-methyl-maspartic acid (NMDA) and metabotropic glutamate 2,3 (mGlur 2,3). However, the role of changes in glutamatergic synaptic markers on energy-dense palatable food preference during development has not been described. Here, we determine the effect of nutritional programing during gestation on fat food choices using a conditioned place preference (CPP) test and an operant training response and its effect on glutamatergic markers in the nucleus accumbens (Nac) shell and prefrontal cortex (PFC). Our data showed that rats displayed preference for palatable fat food and an increase in caloric intake when compared to a chow diet. Notably, 74% of rats showing a preference for fat food intake correlate with a positive HFD-paired score whereas 26% failed to get HFD-conditioned. Also, male rats trained under an operant training response schedule (FR1, FR5 and PR) showed high and low responder groups to work for food. Notably, hypercaloric nutritional programing of female rats leads to exacerbation for reinforcers in female offspring compared to offspring from chow diet. Finally, we found that an operant training response to palatable reinforcers correlates with upregulation of mGlur 2,3 in the NAc shell and PFC of male rats and female offspring. Also, we found selective Nr1 upregulation in NAc shell and the PFC of female offspring. Our data suggest that nutritional programing by hypercaloric intake leads to incentive motivation to work for food and synaptic plasticity alteration in the mesolimbic system.