A mechanism of cyclin D1 action encoded in the patterns of gene expression in human cancer
A mechanism of cyclin D1 action encoded in the patterns of gene expression in human cancer
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DOI：
10.1016/s0092-8674(03)00570-1
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发表时间：
2003-08-08
期刊：
影响因子：
64.5
通讯作者：
Ewen, ME
中科院分区：
文献类型：
--
作者：
Lamb, J;Ramaswamy, S;Ewen, ME
在这里，我们描述了人类肿瘤中的基因表达模式如何被解卷积，以揭示细胞周期蛋白D1癌基因的作用机制。对数百个肿瘤标本中数千个基因的表达模式的计算分析表明，转录因子C/EBPbeta/NF-II6参与了Cyclin Di过度表达的后果。功能分析证实C/EBPbeta参与了受细胞周期蛋白D1影响的基因的调控，并证实该蛋白是细胞周期蛋白D1生物学中一个潜在的重要方面不可或缺的效应因子。这项工作表明，肿瘤基因表达数据库可以用于原位研究人类癌基因的功能。
Here we describe how patterns of gene expression in human tumors have been deconvoluted to reveal a mechanism of action for the cyclin D1 oncogene. Computational analysis of the expression patterns of thousands of genes across hundreds of tumor specimens suggested that a transcription factor, C/EBPbeta/Nf-II6, participates in the consequences of cyclin Di overexpression. Functional analyses confirmed the involvement of C/EBPbeta in the regulation of genes affected by cyclin D1 and established this protein as an indispensable effector of a potentially important facet of cyclin D1 biology. This work demonstrates that tumor gene expression databases can be used to study the function of a human oncogene in situ.