Immunochemical differences between glucocorticoid receptors from corticoid-sensitive and -resistant malignant lymphocytes.
Immunochemical differences between glucocorticoid receptors from corticoid-sensitive and -resistant malignant lymphocytes.
复制标题
皮质激素敏感和耐药恶性淋巴细胞的糖皮质激素受体之间的免疫化学差异。
作者：
J. Stevens;H. Eisen;Y. Stevens;H. Haubenstock;R. Rosenthal;A. Artishevsky
我们探索了利用纯化的大鼠肝脏糖皮质激素受体抗体来研究小鼠和人恶性淋巴细胞糖皮质激素受体免疫化学性质的可能性。为此，将纯化的免疫球蛋白G共价连接到SepharoseCL-4B上。然后，我们检测了该亲和凝胶识别来自小鼠淋巴瘤P1798激素敏感株和耐药株的胞浆[~3H]曲安奈德受体复合体的能力，来自慢性淋巴细胞性白血病患者的CS淋巴细胞，以及来自组织培养中的人白血病淋巴母细胞的CS克隆(CH6)。用小鼠胸腺作为正常CS淋巴细胞糖皮质激素受体的来源。虽然免疫亲和柱保留了CS小鼠和人淋巴细胞特有的58-到62-A(斯托克斯半径)[~3H]曲安奈德受体复合体的70%到84%，但它不能识别存在于糖皮质激素耐药小鼠淋巴瘤P1798细胞中的27-到28-A(斯托克斯半径)糖皮质激素受体。因此，在适当的实验条件下，抗大鼠肝糖皮质激素受体的抗血清与来自不同物种的58-到62-A(斯托克斯半径)糖皮质激素受体之间的交叉反应是可能的。
We have explored the possibility of using antibodies against purified rat liver glucocorticoid receptors to study the immunochemical properties of glucocorticoid receptors from murine and human malignant lymphocytes. For this purpose, purified immune immunoglobulin G was covalently linked to Sepharose CL-4B. We then examined the ability of the affinity gel to recognize cytosolic [3H]triamcinolone acetonide-receptor complexes from the corticoid-sensitive (CS) and -resistant strains of mouse lymphoma P1798, from CS lymphocytes of patients with chronic lymphatic leukemia, and from a CS clone of human leukemic lymphoblasts in tissue culture (CH6). Mouse thymus was used as a source of glucocorticoid receptor from normal CS lymphocytes. Whereas the immunoaffinity column retained 70 to 84% of the 58- to 62-A (Stokes radius) [3H]triamcinolone acetonide-receptor complexes characteristic of the CS mouse and human lymphocytes, it failed to recognize the 27- to 28-A (Stokes radius) glucocorticoid receptor present in corticoid-resistant mouse lymphoma P1798 cells. Therefore, under appropriate experimental conditions, it was possible to demonstrate cross-reactivity between the antiserum against rat liver glucocorticoid receptor and the 58- to 62-A (Stokes radius) glucocorticoid receptor from species as diverse as mouse and humans.