Enumeration of viable Enterococcus faecalis, a predominant apical periodontitis pathogen, using propidium monoazide and quantitative real-time polymerase chain reaction
Enumeration of viable Enterococcus faecalis, a predominant apical periodontitis pathogen, using propidium monoazide and quantitative real-time polymerase chain reaction
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DOI：
10.1111/j.1348-0421.2011.00390.x
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发表时间：
2011-12-01
影响因子：
2.6
通讯作者：
Ansai, Toshihiro
中科院分区：
文献类型：
--
作者：
Takahashi, Yusuke;Yoshida, Akihiro;Ansai, Toshihiro
为了鉴别根尖牙周炎的主要病原菌粪肠球菌（Enterococcus faecalis）的活菌和非活菌，建立了一种结合单叠氮丙啶（PMA）的实时荧光定量PCR方法。PMA对粪肠杆菌细胞无抑菌作用，可对活细胞和非活细胞进行计数。因此，我们分析了9例根尖牙周炎患者根管中的粪肠球菌细胞，以评估该方法的诊断价值。在这些临床标本中成功地区分了活的和非活的粪肠球菌细胞。实时PCR检测结合PMA将有助于根尖牙周炎的准确诊断。
To discriminate between viable and non-viable Enterococcus faecalis, the predominant pathogen in apical periodontitis, a real-time PCR method combined with propidium monoazide (PMA) was developed and evaluated. PMA had no antimicrobial effect on E. faecalis cells and permitted enumeration of both viable and non-viable cells. Therefore, E. faecalis cells from the root canals of nine patients with apical periodontitis were analyzed to evaluate the diagnostic usefulness of this approach. Viable and non-viable E. faecalis cells were successfully discriminated in these clinical specimens. A real-time PCR assay combined with PMA will contribute to the precise diagnosis of apical periodontitis.