Surface determinants of Haemophilus influenzae pathogenicity: comparative virulence of capsular transformants in normal and complement-depleted rats.
Surface determinants of Haemophilus influenzae pathogenicity: comparative virulence of capsular transformants in normal and complement-depleted rats.
复制标题
流感嗜血杆菌致病性的表面决定因素：正常和补体耗尽大鼠中荚膜转化体的毒力比较。
DOI：
10.1093/infdis/148.3.385
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Moxon,ER
中科院分区：
文献类型：
--
作者：
Zwahlen,A;Winkelstein,JA;Moxon,ER
在评估不同荚膜多糖对流感嗜血杆菌致病性的贡献时，比较了流感嗜血杆菌 Rd 菌株和 Rd 菌株的一系列荚膜转化体（b、c、d 和 f 型）在正常和补体缺失大鼠中的毒力。腹膜内接种后，封装的转化体比 Rd 菌株的毒力明显更强，尽管它们的致病性各不相同。 b型毒力最强，c型毒力次之，f型毒力较弱，d型毒力最弱。 C3耗竭增强了大鼠对全身感染的易感性，但不影响转化体的相对毒力。清除率的比较研究表明，b、c 和 f 型转化体在血管内有效存活，但 d 型转化体或 Rd 株则不然； C3耗尽增强了d型的血管内存活率。对荚膜转化体的进一步分析揭示了它们的脂多糖的电泳特性的差异。这些研究表明，独特的荚膜多糖的形成可能不足以解释 b 型流感嗜血杆菌的更强毒力，并且脂多糖可能有助于介导各种血清型的不同致病性。
In an assessment of the contribution of different capsular polysaccharides to the pathogenicity ofHaemophilus influenzae, the virulence ofH influenzaestrain Rd and of a series of capsular transformants (types b, c, d, and f) of strain Rd was compared and in normal and complement-depleted rats. After intraperitoneal inoculation, the encapsulated transformants were strikingly more virulent than strain Rd, although their pathogenicity varied; type b was the most virulent, type c the next most virulent, type f less virulent, and type d the least virulent. C3 depletion enhanced the susceptibility of rats to systemic infection but did not influence the relative virulence of the transformants. Comparative studies of clearance showed efficient intravascular survival of type b, c, and f transformants but not of the type d transformant or strain Rd; C3 depletion enhanced the intravascular survival of type d. Further analysis of the capsular transformants revealed differences in the electrophoretic characteristics of their lipopolysaccharides. These studies indicate that elaboration of a unique capsular polysaccharide may not be a sufficient explanation for the greater virulence ofH influenzaetype b and that lipopolysaccharide may contribute to the mediation of the differential pathogenicity of the various serotypes.