Cholesterol crystallite nucleation in supersaturated model biles from a thermodynamic standpoint.
Cholesterol crystallite nucleation in supersaturated model biles from a thermodynamic standpoint.
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DOI：
10.1016/s0925-4439(02)00098-4
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发表时间：
2002-10
期刊：
影响因子：
--
通讯作者：
Chen-Lun Liu;W. Higuchi
中科院分区：
文献类型：
--
作者：
Chen-Lun Liu;W. Higuchi
A rapid, silicone polymer film uptake method was used to determine the cholesterol (Ch) thermodynamic activity (AT) in taurocholate (TC)–lecithin (L) and taurochenodeoxycholate (TCDC)–L model biles supersaturated with Ch. Also, time-dependent quasielastic light scattering (QLS) measurements and microscopic observations were made to determine the nature of particle species and the Ch nucleation times. In all cases in which Ch–L vesicles were present, a linear relationship between the logarithm of Ch nucleation times and Ch ATwas found. These findings support that Ch ATis the appropriate parameter that represents the Ch nucleation tendency and that vesicles are catalytic sites in the Ch nucleation process. When Ca2+, a nucleation promoter ion, was present in the supersaturated model biles, the increased values of Ch ATquantitatively correlated with shorter Ch nucleation times. These latter findings further demonstrate that Ch ATis the dominant factor in explaining the Ch nucleation tendencies in supersaturated model biles.
