Baicalin improved the spatial learning ability of global ischemia/reperfusion rats by reducing hippocampal apoptosis
Baicalin improved the spatial learning ability of global ischemia/reperfusion rats by reducing hippocampal apoptosis
复制标题
黄芩苷通过减少海马细胞凋亡提高整体缺血/再灌注大鼠的空间学习能力
DOI：
10.1016/j.brainres.2012.06.026
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发表时间：
2012-08-27
期刊：
影响因子：
2.9
通讯作者：
Cheng, Ke
中科院分区：
文献类型：
--
作者：
Cheng, Oumei;Li, Zhenhua;Cheng, Ke
目的：全脑缺血损伤的病理生理机制复杂。近年来的研究表明，脑缺血再灌注引起的过度炎症反应在缺血性损伤中起重要作用。黄芩苷（5，6-dihydroxy-7-O-glucuronide flavonoids glucosides）是从干燥的双子叶黄芩根中提取的黄酮类化合物。黄芩苷在多种脑损伤动物模型中具有神经保护作用，但其机制尚不清楚。我们认为黄芩苷通过抑制炎症反应和减少细胞凋亡来预防急性全脑缺血大鼠空间学习能力的下降。研究方法：将42只Sprague道利大鼠分为3组：假手术（S）组、全脑缺血/再灌注（I/R）组和全脑缺血/再灌注+黄芩苷治疗（I/RB）组。采用Morris水迷宫实验评价大鼠学习记忆能力，HE染色观察海马病理形态学改变，TUNEL法检测海马CA 1区神经元凋亡，Western blot检测海马CA 1区环氧合酶2（考克斯-2）表达。结果：与I/R组相比，黄芩苷能改善I/RB大鼠的学习记忆。I/RB组海马细胞凋亡减少，考克斯-2表达水平降低。此外，I/R组的病理形态学改变比I/RB组更严重。结论：黄芩苷通过抑制考克斯-2表达减少海马细胞凋亡，改善全脑缺血对空间记忆的损害。(C)2012爱思唯尔有限公司版权所有。
Objective: The pathophysiological mechanism of global ischemia damage is complex. Recent studies indicate that the excessive inflammatory response induced by cerebral ischemia/reperfusion plays an important role in ischemic damage. Baicalin (5,6-dihydroxy-7-O-glucuronide flavonoid glycosides) is extracted from the dry dicotyledonous skullcap root, and belongs to one of the flavonoid compounds. Baicalin has a neuroprotective effect in a variety of brain injury animal models, although the mechanism remains unclear. We suggest that baicalin prevents deterioration in rats' spatial learning abilities associated with acute global cerebral ischemia by inhibiting the inflammatory reaction and reducing apoptosis. Methods: Forty-two Sprague Dawley rats were divided into three groups: 14 rats in a sham (S) group; 14 in a global cerebral ischemia/reperfusion (I/R) group; and 14 in a global cerebral ischemia/reperfusion+baicalin treatment (I/RB) group. A Morris water maze test was used to assess learning and memory, HE staining was conducted for pathomorpholog, a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was used to determine neuronal apoptosis, and Western blot for cyclooxygenase-2 (COX-2) expression in the hippocampus CA1 region. Results: Compared to the I/R group, Baicalin improved the learning and memory of I/RB rats. There was decreased hippocampal apoptosis and a reduction in the level of COX-2 expression in the I/RB group. In addition, the pathomorphological changes were worse in the I/R than in the I/RB group. Conclusion: Baicalin improved the detrimental effects on spatial memory associated with global cerebral ischemia by reducing hippocampal apoptosis via the inhibition of COX-2 expression. (C) 2012 Elsevier B.V. All rights reserved.