Generation of gamma irradiation-induced mutant lines of the miniature tomato (Solanum lycopersicum L.) cultivar 'Micro-Tom'
Generation of gamma irradiation-induced mutant lines of the miniature tomato (Solanum lycopersicum L.) cultivar 'Micro-Tom'
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DOI：
10.5511/plantbiotechnology.24.39
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发表时间：
2007-03-01
影响因子：
1.6
通讯作者：
Nishimura, Shigeo
中科院分区：
文献类型：
--
作者：
Matsukura, Chiaki;Yamaguchi, Isao;Nishimura, Shigeo
识别基因及其功能的一种直接方法是基于突变分析的正向遗传方法。为了积累番茄育种和功能基因组学的遗传资源，我们在近交微型矮化品种“Micro-Tom”中通过 300 Gy 的伽马射线照射产生了 6,347 个 M-2 群体品系。根据形态改变和白利糖度畸变，总共筛选了 6,301 个 M-2 品系，并在第一次筛选时选择了 237 个品系作为突变体候选者。随后对自体受精的 M-3 和 M-4 后代进行筛选，产生了 24 个形态突变体系和 11 个异常白利糖度突变体系。分离数据表明，除了两个品系外，大多数突变品系都具有单一隐性突变。萌发M-2幼苗叶绿素突变率为0.37%，实际突变频率为0.5%。选定的突变株系表现出广泛的突变，包括具有严重表型的整株特性，这使得能够更有效地筛选敲除突变体。还描述了三种特征突变体：浅叶、粉红色和短根。
One direct way to identify a gene and its function is a forward genetic approach based on mutation analyses. To accumulate genetic resources for breeding and functional genomics in the tomato, we generated 6,347 lines of an M-2 population with 300 Gy of gamma-ray irradiation in the inbred miniature dwarf variety 'Micro-Tom.' In total, 6,301 M-2 lines were screened based on morphological alteration and brix-aberration, and 237 lines were selected as mutant candidates at the first screening. Subsequent screening of the self-fertilized M-3 and M-4 progeny yielded 24 lines of morphological mutants and 11 lines of aberrant brix mutants. Segregation data suggested that most of the mutant lines had single recessive mutations, with the exception of two lines. The chlorophyll mutation ratio in germinated M-2 seedlings was 0.37% and the actual mutant frequency was 0.5%. The selected mutant lines exhibited a wide range of mutations, including whole plant properties with a severe phenotype, which allowed for more efficient screening of knockout mutants. Three characterized mutants, pale leaf, pink, and short root are also described.