Ciliated epithelial cell lifespan in the mouse trachea and lung
Ciliated epithelial cell lifespan in the mouse trachea and lung
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DOI：
10.1152/ajplung.90209.2008
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发表时间：
2008-07-01
影响因子：
4.9
通讯作者：
Hogan, Brigid L. M.
中科院分区：
文献类型：
--
作者：
Rawlins, Emma L.;Hogan, Brigid L. M.
肺和气管上皮细胞的稳态转换与研究内源性干细胞、操纵体内基因表达或使用病毒载体进行基因治疗的研究人员高度相关。然而，不同气道上皮细胞类型的平均寿命以前没有使用现有的遗传技术进行评估。在这里，我们使用Cre/loxP基因技术对一组小鼠气道中的随机纤毛细胞进行不可磨灭标记，并在体内随访长达18个月。我们证明纤毛气道上皮细胞是一个终末分化群体。此外，它们在气管中的平均半衰期为6个月，在肺中的平均半衰期为17个月，这比以前的估计要长得多，在18个月后仍有大量标记细胞存在。
The steady-state turnover of epithelial cells in the lung and trachea is highly relevant to investigators who are studying endogenous stem cells, manipulating gene expression in vivo, or using viral vectors for gene therapy. However, the average lifetime of different airway epithelial cell types has not previously been assessed using currently available genetic techniques. Here, we use Cre/loxP genetic technology to indelibly label a random fraction of ciliated cells throughout the airways of a cohort of mice and follow them in vivo for up to 18 mo. We demonstrate that ciliated airway epithelial cells are a terminally differentiated population. Moreover, their average half-life of 6 mo in the trachea and 17 mo in the lung is much longer than previously available estimates, with significant numbers of labeled cells still present after 18 mo.