Rapid changes in tubulin RNA synthesis and stability induced by deflagellation in Chlamydomonas.
Rapid changes in tubulin RNA synthesis and stability induced by deflagellation in Chlamydomonas.
复制标题
在衣原体中降临的小管蛋白RNA合成和稳定性的快速变化。
DOI：
10.1083/jcb.99.6.2074
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发表时间：
1984-12
影响因子：
7.8
通讯作者：
Rosenbaum, J L
中科院分区：
文献类型：
--
作者：
Baker, E J;Schloss, J A;Rosenbaum, J L
衣原体鞭毛的脱落诱导微管蛋白和其他鞭毛蛋白的mRNA的快速积累。鞭毛再生过程中α和β微管蛋白RNA合成速率的测量表明，去纤维化使微管蛋白RNA合成快速爆发4-7倍。合成速率在10- 15 min内达到峰值，然后下降回到预爆燃速率。在第一次鞭毛再生完成之前（并且当细胞已经积累了升高水平的微管蛋白RNA时），细胞的再去纤维化诱导了微管蛋白RNA合成的另一次爆发，其在幅度和持续时间上与第一次相同。这一发现表明，诱导信号可能只是简单地重新编程微管蛋白基因的最大合成的瞬时爆发。有证据表明，微管蛋白RNA的稳定性在再生过程中发生变化。稳定性变化包括再生过程中的明显稳定和再生后的加速衰减。
Detachment of the flagella of Chlamydomonas induces a rapid accumulation of mRNAs for tubulin and other flagellar proteins. Measurement of the rate of alpha and beta tubulin RNA synthesis during flagellar regeneration shows that deflagellation elicits a rapid, 4-7- fold burst in tubulin RNA synthesis. The synthesis rate peaks within 10- 15 min, then declines back to the predeflagellation rate. Redeflagellation of cells at times before the first flagellar regeneration is completed (and when cells have already accumulated elevated levels of tubulin RNA) induces another burst in tubulin RNA synthesis which is identical to the first in magnitude and duration. This finding indicates that the induction signal may act to simply reprogram the tubulin genes for a transient burst of maximal synthesis. Evidence is presented that the stability of the tubulin RNAs changes during regeneration. Stability changes include both an apparent stabilization during regeneration and accelerated decay following regeneration.