Revisiting TRPC1 and TRPC6 mechanosensitivity
Revisiting TRPC1 and TRPC6 mechanosensitivity
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DOI：
10.1007/s00424-007-0359-3
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发表时间：
2008-03-01
影响因子：
4.5
通讯作者：
Honore, Eric
中科院分区：
文献类型：
--
作者：
Gottlieb, Philip;Folgering, Joost;Honore, Eric
本文讨论了TRPC1或TRPC6是否是哺乳动物牵张激活的机械敏感性Ca2+渗透性阳离子通道（MscCa）的重要组成部分。我们在非洲绿色猴肾（COS）或中国仓鼠卵巢（CHO）细胞中瞬时表达TRPC 1和TRPC 6，并使用快速压力钳系统监测牵张激活通道的活性。虽然TRPC 1和TRPC 6在蛋白质水平高度表达，但这些亚基的过表达并不显著改变机械敏感电流的幅度。总之，尽管最近提出了几种TRPC通道成员，包括TRPC 1和TRPC 6，在脊椎动物细胞中形成MscCa，但这些TRPC亚基在异源系统中的功能表达仍然存在问题。
This article addresses whether TRPC1 or TRPC6 is an essential component of a mammalian stretch-activated mechano-sensitive Ca2+ permeable cation channel (MscCa). We have transiently expressed TRPC1 and TRPC6 in African green monkey kidney (COS) or Chinese hamster ovary (CHO) cells and monitored the activity of the stretch-activated channels using a fast pressure clamp system. Although both TRPC1 and TRPC6 are highly expressed at the protein level, the amplitude of the mechano-sensitive current is not significantly altered by overexpression of these subunits. In conclusion, although several TRPC channel members, including TRPC1 and TRPC6, have been recently proposed to form MscCa in vertebrate cells, the functional expression of these TRPC subunits in heterologous systems remains problematic.