Effects of protein synthesis on maturation, sperm penetration, and pronuclear development in porcine oocytes
Effects of protein synthesis on maturation, sperm penetration, and pronuclear development in porcine oocytes
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蛋白质合成对猪卵母细胞成熟、精子穿透和原核发育的影响
DOI：
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发表时间：
1992
影响因子：
2.5
通讯作者：
G. Foxcroft
中科院分区：
文献类型：
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作者：
Jianchi Ding;R. Moor;G. Foxcroft
使用体外成熟的猪卵母细胞来测试蛋白质合成对于精子穿透、第二次减数分裂和原核发育的重要性。进行实验以测量蛋白质合成抑制剂（35 μM 或 350 μM 放线菌酮或抑制剂组合）的速率（研究 1）；测试受精过程中蛋白质合成受到抑制时的精子穿透和原核发育（研究 2）；当成熟过程中蛋白质合成受到抑制时，测试卵母细胞减数分裂、精子穿透以及雌性和雄性原核发育（卵母细胞体外成熟，在培养 0、24 或 36 小时添加抑制剂）（研究 3）；并分析这些阶段蛋白质合成模式的变化。精子穿透、卵母细胞减数分裂和雌性原核发育不受受精过程中蛋白质合成完全抑制的影响。相比之下，在成熟过程中抑制蛋白质合成会严重损害减数分裂和原核发育的完成。尽管成熟培养36小时后抑制蛋白质合成并不能完全阻止雄性原核发育(MPN)，但MPN形成率低于对照(52% vs. 72%，P < 0.05)。然而，在解凝后 24 至 36 小时内，蛋白质合成对于 MPN 的形成是绝对必要的。这个成熟时期与卵母细胞中蛋白质重编程的主导阶段一致。 © 1992 Wiley-Liss, Inc.
In vitro matured porcine oocytes were used to test the importance of protein synthesis for sperm penetration, the second meiotic division, and pronuclear development. Experiments were carried out to measure rates of protein synthesis inhibitors (35 μM or 350 μM cycloheximide or a combination of inhibitors) (study 1); to test for sperm penetration and pronuclear development when protein synthesis was inhibited during fertilization (study 2); to test for oocyte meiosis, sperm penetration, and female and male pronuclear development when protein synthesis was inhibited during maturation (oocyte maturation in vitro with addition of inhibitor at 0, 24, or 36 hr of culture) (study 3); and to analyze the changes in the pattern of protein synthesis during these phases. Sperm penetration, oocyte meiosis, and female pronuclear development were not affected by the total inhibition of protein synthesis during fertilization. By contrast, inhibiting protein synthesis during maturation severely impaired the completion of meiosis and pronuclear development. Although inhibition of protein synthesis after 36 hr of maturation culture did not totally block male pronuclear development (MPN), the rate of MPN formation was lower than for controls (52% vs. 72%, P < 0.05). However, protein synthesis was absolutely essential between 24 and 36 hr for the formation of MPN after decondensation. This period of maturation coincided with the dominant phase of protein reprogramming in the oocyte. © 1992 Wiley‐Liss, Inc.