De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns
De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns
复制标题
DOI：
10.1038/s41467-019-12932-w
复制
发表时间：
2019-10-31
影响因子：
16.6
通讯作者：
Danelon, Christophe
中科院分区：
文献类型：
--
作者：
Godino, Elisa;Lopez, Jonas Noguera;Danelon, Christophe
Min生化网络调节细菌细胞分裂，是自组织分子系统的典型例子。依赖于纯化蛋白质的无细胞测定表明，MinE和MinD自组织成表面波和振荡模式。在从基本生物模块开发合成细胞的背景下，利用Min振荡可能使我们能够实现高阶细胞功能。为了传递遗传信息，Min系统必须编码在一个可以复制、转录和翻译的DNA分子中。在这里，MinD和MinE蛋白质从脂质体内的基因重新合成。观察到动态蛋白质模式和伴随的脂质体形状变形。当与细胞骨架蛋白FtsA和FtsZ整合时，合成Min系统能够动态调节FtsZ模式。通过对Min蛋白自组织和膜重塑进行遗传控制，我们的方法为体外细菌分裂过程的定向进化提供了独特的机会。
The Min biochemical network regulates bacterial cell division and is a prototypical example of self-organizing molecular systems. Cell-free assays relying on purified proteins have shown that MinE and MinD self-organize into surface waves and oscillatory patterns. In the context of developing a synthetic cell from elementary biological modules, harnessing Min oscillations might allow us to implement higher-order cellular functions. To convey hereditary information, the Min system must be encoded in a DNA molecule that can be copied, transcribed, and translated. Here, the MinD and MinE proteins are synthesized de novo from their genes inside liposomes. Dynamic protein patterns and accompanying liposome shape deformation are observed. When integrated with the cytoskeletal proteins FtsA and FtsZ, the synthetic Min system is able to dynamically regulate FtsZ patterns. By enabling genetic control over Min protein self-organization and membrane remodeling, our methodology offers unique opportunities towards directed evolution of bacterial division processes in vitro.