Functional Analysis of Phosphate Transporter OsPHT4 Family Members in Rice
Functional Analysis of Phosphate Transporter OsPHT4 Family Members in Rice
复制标题
水稻磷酸盐转运蛋白 OsPHT4 家族成员的功能分析
DOI：
10.1016/j.rsci.2020.09.006
复制
发表时间：
2020-11-01
期刊：
影响因子：
4.8
通讯作者：
Zhao Hongyu
中科院分区：
文献类型：
--
作者：
Li Ruili;Wang Jiaoling;Zhao Hongyu
磷酸盐在细胞质和亚细胞区室之间的运输对于植物代谢调节至关重要。我们进行了生物信息学分析、酵母异源表达、基因表达模式和亚细胞定位分析来表征OsPHT4基因家族在水稻中的可能功能。与高等植物的 PHT4 基因一起，OsPHT4 可以分为六个不同的组。 OsPHT4;1-OsPHT4;4 定位于叶绿体，OsPHT4;6-1 和 OsPHT4;6-2 定位于高尔基体。 OsPHT4 蛋白可以介导酵母中无机磷酸盐 (Pi) 的运输。此外，qRT-PCR 测定的动态转录变化揭示了 OsPHT4 基因响应磷酸盐饥饿、水杨酸、脱落酸和盐胁迫处理的不同表达谱。这些结果表明，OsPHT4 蛋白参与细胞质和叶绿体或高尔基体之间的 Pi 分配，并且还参与应激反应。
The transport of phosphate between cytoplasm and subcellular compartments is critical for plant metabolic regulation. We conducted bioinformatic analysis, heterologous expression in yeast, gene expression pattern and subcellular localization analysis to characterize the possible functions of OsPHT4 gene family in rice. Together with the PHT4 genes from higher plants, OsPHT4s can be classified into six distinct groups. OsPHT4;1-OsPHT4;4 are targeted to chloroplasts, and OsPHT4;6-1 and OsPHT4;6-2 are located to Golgi apparatus. OsPHT4 proteins can mediate inorganic phosphate (Pi) transport in yeast. In addition, dynamic transcriptional changes determined by qRT-PCR revealed different expression profiles of OsPHT4 genes in response to phosphate starvation, salicylic acid, abscisic acid and salt stress treatments. These results suggested that OsPHT4 proteins are involved in Pi distribution between the cytoplasm and chloroplast or Golgi apparatus and also involved in stress responses.