Identification of Campylobacter jejuni Genes Involved in Its Interaction with Epithelial Cells
Identification of Campylobacter jejuni Genes Involved in Its Interaction with Epithelial Cells
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DOI：
10.1128/iai.00109-10
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发表时间：
2010-08-01
影响因子：
3.1
通讯作者：
Galan, Jorge E.
中科院分区：
文献类型：
--
作者：
Novik, Veronica;Hofreuter, Dirk;Galan, Jorge E.
空肠弯曲菌是工业化国家传染性胃肠炎的主要原因。其进入非吞噬细胞并在其中存活的能力被认为是发病机制中非常重要的。然而，人们对C.介导这些过程的空肠决定簇。通过广泛的转座子诱变筛选，我们已经确定了几个基因座所需的C。空肠上皮细胞内的有效进入和存活。在这些位点中，aspA、aspB和sodB的插入突变导致C的急剧降低。空肠进入和/或在宿主细胞内存活，以及在动物模型中定植的严重缺陷。这些发现对理解C. jejuni宿主细胞的相互作用进行了讨论。
Campylobacter jejuni is the leading cause of infectious gastroenteritis in industrialized nations. Its ability to enter and survive within nonphagocytic cells is thought to be very important for pathogenesis. However, little is known about the C. jejuni determinants that mediate these processes. Through an extensive transposon mutagenesis screen, we have identified several loci that are required for C. jejuni efficient entry and survival within epithelial cells. Among these loci, insertional mutations in aspA, aspB, and sodB resulted in drastic reduction in C. jejuni entry and/or survival within host cells and a severe defect in colonization in an animal model. The implications of these findings for the understanding of C. jejuni-host cell interactions are discussed.