Evaluation of the Molecular Interaction Between CCN Protein and Its Binding Partners: A Solid-Phase Binding Assay and Surface Plasmon Resonance
Evaluation of the Molecular Interaction Between CCN Protein and Its Binding Partners: A Solid-Phase Binding Assay and Surface Plasmon Resonance
复制标题
CCN 蛋白与其结合配偶体之间分子相互作用的评估：固相结合测定和表面等离子共振
DOI：
10.1007/978-1-0716-2744-0_7
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发表时间：
2022
影响因子：
--
通讯作者：
Takigawa Masaharu
中科院分区：
文献类型：
--
作者：
Aoyama Eriko;Takigawa Masaharu
已知CCN蛋白可与多种生长因子、细胞因子和膜蛋白结合。由于这些结合与CCN蛋白的功能密切相关，因此分析结合伙伴是了解CCN蛋白作用机制的第一步。本章描述了用于这种分析的两种方法：固相结合分析法，由于其简单和成本优势，适合于容易地确认结合，以及表面等离子体共振分析，可以确定CCN蛋白与其伙伴之间的结合亲和力。
CCN proteins are known to bind to various growth factors, cytokines, and membrane proteins. Since these bindings are closely involved in the function of CCN proteins, the analysis of the binding partners is the first step toward understanding the mechanisms of actions of CCN proteins. This chapter describes two approaches used for such analyses: a solid-phase binding assay, which is suitable for confirming the binding easily because of its simplicity and cost advantage, and a surface plasmon resonance assay, which can determine the binding affinities between CCN proteins and their partners.