Chemical properties of egg-mass mucin complexes of the ommastrephid squid Todarodes pacificus
Chemical properties of egg-mass mucin complexes of the ommastrephid squid Todarodes pacificus
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DOI：
10.1093/mollus/70.2.117
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发表时间：
2004-05-01
影响因子：
1.2
通讯作者：
Sakurai, Y
中科院分区：
文献类型：
--
作者：
Kimura, S;Higuchi, Y;Sakurai, Y
本文描述了日本太平洋褶柔鱼（Todarodes pacificus，Steenstrup，1880）卵块糖蛋白的性质，并提供了一种在卵块形成过程中起作用的化学证据。从圈养产卵的卵块的表层和内部果冻中收集样品，并将内部果冻分离成原纤维和果冻的液体部分。然后分离出成熟鱿鱼的卵囊和输卵管腺中的粘液物质，并与卵块各部分的粘液物质进行比较。表面层和内部纤维被发现是由不同类型的粘蛋白复合物和起源于水溶性和不溶性部分nidamental mucocystent，分别。然而，输卵管粘液物质似乎在原纤维的形成中没有发挥作用。这些结果表明，卵块表面层是来自于水溶性部分的nidamental mucosystem，和原纤维内的质量是来自于不溶性部分的mucosystem。
This paper describes the properties of egg-mass glycoproteins from the Japanese common squid Todarodes pacificus (Steenstrup, 1880) and provides chemical evidence for the role of a nidamental mucosubstance in the formation of egg masses. Samples were collected from the surface layer and the interior jelly of an egg mass spawned in captivity, and the interior jelly was separated into fibrils and the liquid portion of the jelly. Mucosubstances in the nidamental and oviducal glands of maturing squid were then isolated and compared with those of each part of the egg mass. The surface layer and the interior fibrils were found to be composed of distinct types of mucin complexes and to have originated from the water-soluble and insoluble fractions of nidamental mucosubstance, respectively. Oviducal mucosubstance, however, appeared to play no role in the formation of the fibrils. These results suggest the egg-mass surface layer is derived from the water-soluble fraction of the nidamental mucosubstance, and the fibrils within the mass are derived from the insoluble fraction of the mucosubstance.