A Broad Role for Cysteines in Bovine Antibody Diversity.
A Broad Role for Cysteines in Bovine Antibody Diversity.
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DOI：
10.4049/immunohorizons.1900058
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发表时间：
2019-10-16
期刊：
影响因子：
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通讯作者：
Smider, Vaughn V
中科院分区：
文献类型：
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作者：
Haakenson, Jeremy K;Deiss, Thaddeus C;Smider, Vaughn V
大多数脊椎动物中的Ab多样性是由通过V（D）J重组形成的CDR环上的氨基酸侧链的分类引起的。牛（普通牛）有一个低的组合多样性的潜力，因为少数高度同源的V，D和J基因片段。尽管如此，Ab库的一个子集（10%）含有异常长的CDR H链（HC）3（H3）区，具有丰富的半胱氨酸和二硫键环多样性，通过单一的V-D-J重组事件，然后大量的体细胞超突变而多样化。然而，编码较短CDR H3的库的大得多的部分尚未详细检查。种系基因片段的分析揭示了HC V区中的非典型半胱氨酸和HC D区中的显著半胱氨酸含量。天然存在的较短CDR H3的深度测序分析（
Ab diversity in most vertebrates results from the assortment of amino acid side chains on CDR loops formed through V(D)J recombination. Cows (Bos taurus) have a low combinatorial diversity potential because of a small number of highly homologous V, D, and J gene segments. Despite this, a subset of the Ab repertoire (10%) contains exceptionally long CDR H chain (HC) 3 (H3) regions with a rich diversity of cysteines and disulfide-bonded loops that diversify through a single V-D-J recombination event followed by massive somatic hypermutation. However, the much larger portion of the repertoire, encoding shorter CDR H3s, has not been examined in detail. Analysis of germline gene segments reveals noncanonical cysteines in the HC V regions and significant cysteine content in the HC D regions. Deep sequencing analysis of naturally occurring shorter CDR H3 (