Association of Borrelia garinii and B-valaisiana with songbirds in Slovakia
Association of Borrelia garinii and B-valaisiana with songbirds in Slovakia
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DOI：
10.1128/aem.69.5.2825-2830.2003
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发表时间：
2003-05-01
影响因子：
4.4
通讯作者：
Kurtenbach, K
中科院分区：
文献类型：
--
作者：
Hanincová, K;Taragelová, V;Kurtenbach, K
在欧洲，伯氏疏螺旋体的11个基因种中有6个在寻找蓖麻硬蜱时流行。在中欧的大部分地区，B.afzelii、B.garinii和B.valaisiana是最常见的种，而B.burgdorferi Sensu Stru Strto、B.Bissettii和B.lusitaniae很少见。此前，已有研究表明，阿夫泽利氏杆菌与欧洲啮齿动物有关。因此，本研究的目的是确定斯洛伐克的加里尼氏杆菌和瓦莱西亚氏杆菌的宿主。在布拉迪斯拉发附近的一片林地里捕获了鸣禽，并对其进行了充血的扁虱调查。L等人在同一地区采集到了蓖麻黄粉虱。通过聚合酶链式反应、DNA-DNA杂交法和核苷酸测序法对两个硬壳库进行了螺旋体感染分析。在捕获的17种鸣鸟中，有3种感染了螺旋体感染的扁虱。在以鸟类为食的硬蜱中，螺旋体被分型为加里氏双歧杆菌和瓦氏双歧杆菌，而在猎食的硬蜱中感染了阿氏双歧杆菌、加里尼双歧杆菌和巴氏双歧杆菌。此外，在以鸟为食的扁虱和探寻的扁虱中，也发现了相同的加里尼芽胞杆菌OspA等位基因。结果表明，鸣禽是加里氏杆菌和巴氏杆菌的寄主，而不是阿维氏杆菌的宿主。这项研究和以前的研究证实，伯氏杆菌是与宿主相关的，这种细菌物种复合体包含不同的生态型。
In Europe, 6 of the 11 genospecies of Borrelia burgdorferi sensu lato are prevalent in questing Ixodes ricinus ticks. In most parts of Central Europe, B. afzelii, B. garinii, and B. valaisiana are the most frequent species, whereas B. burgdorferi sensu stricto, B. bissettii, and B. lusitaniae are rare. Previously, it has been shown that B. afzelii is associated with European rodents. Therefore, the aim of this study was to identify reservoir hosts of B. garinii and B. valaisiana in Slovakia. Songbirds were captured in a woodland near Bratislava and investigated for engorged ticks. Questing L ricinus ticks were collected in the same region. Both tick pools were analyzed for spirochete infections by PCR, followed by DNA-DNA hybridization and, for a subsample, by nucleotide sequencing. Three of the 17 captured songbird species were infested with spirochete-infected ticks. Spirochetes in ticks that had fed on birds were genotyped as B. garinii and B. valaisiana, whereas questing ticks were infected with B. afzelii, B. garinii, and B. valaisiana. Furthermore, identical ospA alleles of B. garinii were found in ticks that had fed on the birds and in questing ticks. The data show that songbirds are reservoir hosts of B. garinii and B. valaisiana but not of B. afzelii. This and previous studies confirm that B. burgdorferi sensu lato is host associated and that this bacterial species complex contains different ecotypes.