The human intestinal cell lines Caco-2 and LS174T as models to study cell-type specific mucin expression
The human intestinal cell lines Caco-2 and LS174T as models to study cell-type specific mucin expression
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DOI：
10.1007/bf00702340
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发表时间：
1996-10-01
影响因子：
3
通讯作者：
Einerhand, AWC
中科院分区：
文献类型：
--
作者：
VanKlinken, BJW;Oussoren, E;Einerhand, AWC
研究了肠上皮样Caco-2和杯状细胞样LS174T细胞系在增殖分化过程中粘蛋白的表达。Caco-2细胞表达MUC1、MUC3、MUC4和MUC5A/C的mRNAs，而MUC2和MUC6mRNAs几乎不表达。此外，MUC3mRNA的表达依赖于分化，就像肠细胞一样。同时在Caco-2细胞中检测到MUC3蛋白前体(约550 kDa)。在LS174T细胞中，MUC1、MUC2和MUC6的粘蛋白mRNAs呈高水平表达，而MUC3、MUC4和MUC5A/C的mRNAs呈低水平表达。在蛋白质水平上，LS174T细胞表达杯状细胞特异性粘蛋白前体MUC2、MUC5A/C和MUC6，表观分子量分别约为600 kDa、470/500 kDa和400 kDa。未检测到MUC3蛋白。此外，人胆囊粘蛋白(类似于470 kDa的前体)在LS174T细胞中表达，其基因尚未被鉴定。此外，LS174T细胞还能合成和分泌杯状细胞特异性成熟MUC2、MUC5A/C和人胆囊黏蛋白。结论：Caco-2和LS174T细胞系为阐明粘蛋白表达的细胞类型特异性机制提供了良好的体外模型。
Mucin expression was studied during proliferation and differentiation of the enterocyte-like Caco-2 and goblet cell-like LS174T cell lines. Caco-2 cells express mRNAs of MUC1, MUC3, MUC4 and MUC5A/C whereas MUC2 and MUC6 mRNAs are virtually absent. Furthermore, MUC3 mRNA is expressed in a differentiation dependent manner, as is the case for enterocytes. Concomitantly MUC3 protein precursor (similar to 550 kDa) was detected in Caco-2 cells. In LS174T cells mucin mRNAs of MUC1, MUC2 and MUC6 are constitutively expressed at high levels, whereas MUC3, MUC4 and MUC5A/C mRNAs are present at low levels. At the protein level LS174T cells express the goblet cell specific mucin protein precursors MUC2, MUC5A/C and MUC6 with apparent molecular masses of about 600 kDa, 470/500 kDa and 400 kDa respectively. MUC3 protein is not detectable. Furthermore, human gallbladder mucin protein (similar to 470 kDa precursor), of which the gene has not yet been identified, is expressed in LS174T cells. In addition, synthesis and secretion of the goblet cell specific mature MUC2, MUC5A/C and human gallbladder mucin was demonstrated in LS174T cells. It is concluded that Caco-2 and LS174T cell lines provide excellent in vitro models to elucidate the cell-type specific mechanisms responsible for mucin expression.