Drug resistance mutations in the HIV-1 protease and reverse transcriptase genes in antiretroviral-naive Vietnamese children
Drug resistance mutations in the HIV-1 protease and reverse transcriptase genes in antiretroviral-naive Vietnamese children
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未接受过抗逆转录病毒治疗的越南儿童中 HIV-1 蛋白酶和逆转录酶基因的耐药性突变
DOI：
10.1089/aid.2011.0280
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Trinh QD
中科院分区：
文献类型：
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作者：
Yeasmin L;Akter S;Islam AMS;Rahman MM;M Moroi;Jesmin S;Trinh QD
最近，抗艾滋病毒药物已可用于治疗越南感染艾滋病毒的儿童；然而，未接受过抗逆转录病毒治疗的儿童中 HIV-1 耐药性的遗传背景仍有待研究。 2004年至2005年期间，从越南南部各省未接受过抗逆转录病毒治疗并在胡志明市第一儿童医院住院的儿童中分离出104株HIV-1 CRF01-AE亚型毒株，其中79株用于蛋白酶和逆转录酶（RT）基因的扩增和序列分析。蛋白酶基因中发现了轻微突变，包括L10I、I13V、G16E、M36I、D60E、I62V、I64V、L63P、H69K、V82I和I93L。在这些突变中，在所有研究的菌株中均检测到了 M36I 和 H69K。然而，蛋白酶基因中的所有氨基酸变化都被认为是多态性。在RT基因中，6个菌株检测到3个主要突变：1个菌株中V75M突变，2个菌株中Y181C突变，3个菌株中M184I突变。本研究评估的所有药物和药物类别的原发性或传播性 HIV 耐药率为 7.6%。这些发现为越南的抗逆转录病毒治疗提供了有用的背景，并为全球艾滋病毒耐药性监测提供了参考数据。这项研究表明，越南的 HIVDR 患病率最近可能有所上升。越南艾滋病毒耐药性监测是必要的。
Anti-HIV drugs have recently become available for the treatment of children infected with HIV in Vietnam; however, the genetic background of HIV-1 drug resistance in antiretroviral-naive children has yet to be studied. Of the 104 HIV-1 CRF01-AE subtype strains that were previously isolated from antiretroviral-naive children from the provinces of southern Vietnam and hospitalized in Children Hospital 1 in Ho Chi Minh City from 2004 to 2005, 79 strains were used for amplification and sequence analyses of the protease and reverse transcriptase (RT) genes. Minor mutations were found in the protease gene, including L10I, I13V, G16E, M36I, D60E, I62V, I64V, L63P, H69K, V82I, and I93L. Of these mutations, M36I and H69K were detected in all of the strains that were studied. However, all of the amino acid changes in the protease gene were considered to be polymorphisms. In the RT gene, three major mutations were detected in six strains: the V75M mutation in one strain, the Y181C mutation in two strains, and the M184I mutation in three strains. The prevalence of primary or transmitted HIV drug resistance to all of the drugs and drug classes that were evaluated in this study was 7.6%. These findings provide a useful background for antiretroviral therapy in Vietnam and contribute reference data for the surveillance of HIV drug resistance around the world. This study suggests that the prevalence of HIVDR in Vietnam may have recently increased. The monitoring of HIV drug resistance in Vietnam is necessary.