Convergent antibody responses to the SARS-CoV-2 spike protein in convalescent and vaccinated individuals.
Convergent antibody responses to the SARS-CoV-2 spike protein in convalescent and vaccinated individuals.
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DOI：
10.1016/j.celrep.2021.109604
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发表时间：
2021-08-24
期刊：
影响因子：
8.8
通讯作者：
Crowe JE Jr
中科院分区：
文献类型：
--
作者：
Chen EC;Gilchuk P;Zost SJ;Suryadevara N;Winkler ES;Cabel CR;Binshtein E;Chen RE;Sutton RE;Rodriguez J;Day S;Myers L;Trivette A;Williams JK;Davidson E;Li S;Doranz BJ;Campos SK;Carnahan RH;Thorne CA;Diamond MS;Crowe JE Jr
不相关的个体可以产生基因相似的抗体克隆，称为公共克隆型，这已经在对不同传染病的反应中看到，以及健康个体。在这里，我们确定了37个公共克隆型的记忆B细胞从严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）感染的恢复期幸存者或浆母细胞从接种后与mRNA编码的刺突蛋白的个人。我们确定了29个公共克隆型，包括识别刺突蛋白S1亚基中的受体结合结构域（RBD）的克隆（包括一个中和的、血管紧张素转换酶2 [ACE 2]阻断的克隆，该克隆在体内起保护作用）和其他识别结合S2结构域的非RBD表位的克隆。一些公共克隆型的种系回复突变体形式有效地结合刺突蛋白，表明这些常见的种系编码的抗体被预先配置用于亲和识别。大量公共克隆型的鉴定为SARS-CoV-2疫苗有效性的分子基础提供了深入了解，并揭示了驱动常见病毒逃逸突变体选择的免疫压力。Chen等人描述了在接种疫苗和自然感染的个体中引起的SARS-CoV-2峰值的中和和非中和公共抗体。一些公共克隆型的种系回复突变体形式有效地结合到刺突蛋白，这表明共同的种系编码的识别特性。
Unrelated individuals can produce genetically similar clones of antibodies, known as public clonotypes, which have been seen in responses to different infectious diseases, as well as healthy individuals. Here we identify 37 public clonotypes in memory B cells from convalescent survivors of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection or in plasmablasts from an individual after vaccination with mRNA-encoded spike protein. We identify 29 public clonotypes, including clones recognizing the receptor-binding domain (RBD) in the spike protein S1 subunit (including a neutralizing, angiotensin-converting enzyme 2 [ACE2]-blocking clone that protects in vivo) and others recognizing non-RBD epitopes that bind the S2 domain. Germline-revertant forms of some public clonotypes bind efficiently to spike protein, suggesting these common germline-encoded antibodies are preconfigured for avid recognition. Identification of large numbers of public clonotypes provides insight into the molecular basis of efficacy of SARS-CoV-2 vaccines and sheds light on the immune pressures driving the selection of common viral escape mutants. Chen et al. describe neutralizing and non-neutralizing public antibodies elicited to SARS-CoV-2 spike in vaccinated and naturally infected individuals. Germline-revertant forms of some public clonotypes bind efficiently to spike protein, suggesting common germline-encoded properties for recognition.
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影响因子：
8
作者：
Bailey, Justin R.;Flyak, Andrew I.;Crowe, James E., Jr.
通讯作者：
Crowe, James E., Jr.
影响因子：
30.3
作者：
Case, James Brett;Rothlauf, Paul W.;Whelan, Sean P. J.
通讯作者：
Whelan, Sean P. J.
影响因子：
48
作者：
Bepler, Tristan;Morin, Andrew;Berger, Bonnie
通讯作者：
Berger, Bonnie
影响因子：
3.7
作者：
Case, James Brett;Bailey, Adam L.;Diamond, Michael S.
通讯作者：
Diamond, Michael S.
SARS-COV-2和SARS-COV之间的自然突变决定了交叉反应抗体中和。
影响因子：
6.7
作者：
Wu NC;Yuan M;Bangaru S;Huang D;Zhu X;Lee CD;Turner HL;Peng L;Yang L;Burton DR;Nemazee D;Ward AB;Wilson IA
通讯作者：
Wilson IA