The inhibitory mechanism of methyl jasmonate on Aspergillus flavus growth and aflatoxin biosynthesis and two novel transcription factors are involved in this action
The inhibitory mechanism of methyl jasmonate on Aspergillus flavus growth and aflatoxin biosynthesis and two novel transcription factors are involved in this action
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DOI：
10.1016/j.foodres.2020.110051
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发表时间：
2021-02-01
影响因子：
8.1
通讯作者：
Xing, Fuguo
中科院分区：
文献类型：
--
作者：
Li, Xu;Ren, Yaoyao;Xing, Fuguo
黄曲霉毒素B-1（AFB（1））是毒性最强、致癌性最高的真菌毒素，其生物合成依赖于一系列酶促反应和复杂的调控系统。茉莉酸甲酯（MeJA）是一种与逆境胁迫相关的植物激素。本研究中，MeJA对A.黄曲霉生长和黄曲霉毒素B（1）的产生具有剂量依赖性。扫描电镜和透射电镜分析表明，在不同的温度下，A.用MeJA处理的黄色。RNA-Seq结果表明，在不同的黄曲霉毒素（AFs）基因表达水平上，初始阶段差异不显著，而中后期阶段差异显著，这可能是由于全局调控因子的减少，尤其是AtfB的减少。更重要的是，两个新的调控因子（AFLA_085880和AFLA_015850）参与了该抑制，并被认为是AFs产生的关键正调控因子。这两个基因突变体还显示AFs簇基因和AFs相关调节基因的表达显著降低，随后的AFB（1）生物合成也显著降低。本研究部分阐明了MeJA的抑制机制，为消除AFs污染提供了依据。
As the most toxic and carcinogenic mycotoxin, aflatoxin B-1 (AFB(1)) biosynthesis depends on a series of enzymatic reactions and a complicated regulatory system. Methyl jasmonate (MeJA) is one of stress associated phytohormones. In this study, MeJA could inhibit A. flavus growth and AFB(1) production with a dose-dependent manner. SEM and TEM analysis indicated that morphological ultrastructure deteriorations were observed in A. flavus treated with MeJA. RNA-Seq indicated that the initial-steps aflatoxins (AFs) genes were no drastic difference, but the middle- and later-steps genes were significantly down-regulated, which might be due to the decreases of global regulators, especially AtfB. More importantly, two novel regulators (AFLA_085880 and AFLA_015850) were involved in the inhibition, and were recognized as the critically positive regulators for AFs productions. The two genes mutants also showed significantly decrease expressions of AFs cluster genes and AFs associated regulators, and subsequent AFB(1) biosynthesis. This research partly clarified inhibitory mechanism of MeJA and made some contributions to the elimination of AFs contamination.