Dysregulated cholinergic network as a novel biomarker of poor prognostic in patients with head and neck squamous cell carcinoma.
Dysregulated cholinergic network as a novel biomarker of poor prognostic in patients with head and neck squamous cell carcinoma.
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DOI：
10.1186/s12885-015-1402-y
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发表时间：
2015-05-10
期刊：
影响因子：
3.8
通讯作者：
Cabezas-Herrera J
中科院分区：
文献类型：
--
作者：
Castillo-González AC;Nieto-Cerón S;Pelegrín-Hernández JP;Montenegro MF;Noguera JA;López-Moreno MF;Rodríguez-López JN;Vidal CJ;Hellín-Meseguer D;Cabezas-Herrera J
在呼吸道中，胆碱能激动剂通过激活烟碱和毒扁豆碱受体直接发挥增殖作用。肿瘤如何对异常激活的胆碱能信号做出反应是吸烟相关癌症的关键问题。这项研究旨在探索胆碱能信号变化与癌症生物学的可能联系。比较了57对头颈部鳞状细胞癌(HNSCC)和癌旁非癌组织(ANCT)中ACh相关蛋白的mRNA水平和ACh水解酶活性。ANCT中乙酰胆碱酯酶(AChE)和丁酰胆碱酯酶(BChE)活性(分别为5.416 ± 0.501 mU/mg蛋白和6.350 ± 0.599 mU/mg蛋白)的测定表明，上呼吸道能够控制ACh的可利用性。HNSCC中乙酰胆碱酯酶(AchE)和胆碱酯酶(BChE)活性分别降至3.584 ± 0.599 mU/mg蛋白(p = 0.002)和3.965 ± 0.423 mU/mg蛋白(p <； 0.001)。此外，低AChE活性和高BChE活性的肿瘤与较短的患者总生存期相关。鼻咽癌和鼻咽癌AchE-T、α3、α5、α9和β2亚单位的基因表达水平存在差异。烟草暴露对AChE-H和AChE-TmRNAs的表达均有较大影响。未受影响的和癌变的片段含有主要的AChE二聚体和BChE四聚体。在HNSCC中，缺乏神经源性原发联系的AChE与神经末梢重塑和丢失的病理结果一致。我们的结果表明，HNSCC中低AChE活性可以用来预测头颈癌患者的生存。因此，ChE活性水平可作为判断预后的可靠指标。本文的在线版本(doi：10.1186/s12885-0151402-y)包含补充材料，授权用户可以使用。
In airways, a proliferative effect is played directly by cholinergic agonists through nicotinic and muscarinic receptors activation. How tumors respond to aberrantly activated cholinergic signalling is a key question in smoking-related cancer. This research was addressed to explore a possible link of cholinergic signalling changes with cancer biology. Fifty-seven paired pieces of head and neck squamous cell carcinoma (HNSCC) and adjacent non-cancerous tissue (ANCT) were compared for their mRNA levels for ACh-related proteins and ACh-hydrolyzing activity. The measurement in ANCT of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities (5.416 ± 0.501 mU/mg protein and 6.350 ± 0.599 mU/mg protein, respectively) demonstrated that upper respiratory tract is capable of controlling the availability of ACh. In HNSCC, AChE and BChE activities dropped to 3.584 ± 0.599 mU/mg protein (p = 0.002) and 3.965 ± 0.423 mU/mg protein (p < 0.001). Moreover, tumours with low AChE activity and high BChE activity were associated with shorter patient overall survival. ANCT and HNSCC differed in mRNA levels for AChE-T, α3, α5, α9 and β2 for nAChR subunits. Tobacco exposure had a great impact on the expression of both AChE-H and AChE-T mRNAs. Unaffected and cancerous pieces contained principal AChE dimers and BChE tetramers. The lack of nerve-born PRiMA-linked AChE agreed with pathological findings on nerve terminal remodelling and loss in HNSCC. Our results suggest that the low AChE activity in HNSCC can be used to predict survival in patients with head and neck cancer. So, the ChE activity level can be used as a reliable prognostic marker. The online version of this article (doi:10.1186/s12885-015-1402-y) contains supplementary material, which is available to authorized users.
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影响因子：
4.2
作者：
Darreh-Shori, Taher;Vijayaraghavan, Swetha;Nordberg, Agneta
通讯作者：
Nordberg, Agneta
抗癌药物诱导细胞凋亡过程中 c-Jun-NH2 末端激酶通路介导的乙酰胆碱酯酶表达
影响因子：
8
作者：
Deng, R.;Li, W.;Zhu, X-F
通讯作者：
Zhu, X-F
影响因子：
6.1
作者：
Girard, Emmanuelle;Bernard, Veronique;Molgo, Jordi
通讯作者：
Molgo, Jordi
影响因子：
158.5
作者：
Bernier, J;Domenge, C;van Glabbeke, M
通讯作者：
van Glabbeke, M
DOI：
10.2463/mrms.1.157
发表时间：
2002-11-01
期刊：
Magnetic resonance in medical sciences : MRMS : an official journal of Japan Society of Magnetic Resonance in Medicine
影响因子：
--
作者：
Kato, Koki;Tomura, Noriaki;Watarai, Jiro
通讯作者：
Watarai, Jiro