Enhancement by adriamycin of the effects of 12-O-tetradecanoylphorbol-13-acetate on mouse epidermal glutathione peroxidase activity, ornithine decarboxylase induction and skin tumor promotion.
Enhancement by adriamycin of the effects of 12-O-tetradecanoylphorbol-13-acetate on mouse epidermal glutathione peroxidase activity, ornithine decarboxylase induction and skin tumor promotion.
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阿霉素增强 12-O-十四烷酰佛波醇-13-乙酸酯对小鼠表皮谷胱甘肽过氧化物酶活性、鸟氨酸脱羧酶诱导和皮肤肿瘤促进的作用。
DOI：
10.1016/0304-3835(85)90151-x
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发表时间：
1985
期刊：
影响因子：
9.7
通讯作者：
E. Perchellet
中科院分区：
文献类型：
--
作者：
J. Perchellet;G. S. Kishore;E. Perchellet
阿霉素（ADR）在体内外均不能抑制12-O-十四酰佛波醇-13-乙酸酯（TPA）对小鼠表皮的生物学作用，反而增强TPA的生物学作用。在存在ADR的情况下，肿瘤促进剂引起谷胱甘肽（GSH）过氧化物酶（GSH：H2 O2氧化还原酶，EC 1.11.1.9）活性的更大连续快速增加和长期降低，伴随着分离的表皮细胞中还原型（GSH）/氧化型（GSSG）谷胱甘肽比率的更大降低。ADR消耗细胞内GSH水平的能力与其增加基础和TPA诱导的鸟氨酸脱羧酶（ODC，l-鸟氨酸羧化酶，EC 4.1.1.17）活性的能力相关。在体内，局部ADR治疗也增强TPA诱导的ODC活性以及TPA在小鼠皮肤癌变的两阶段系统中的促肿瘤能力。由于脂质过氧化作用与ADR毒性有关，这些数据表明，ADR对TPA促肿瘤能力的增强可能是增加氧化挑战的结果，这种挑战破坏了表皮细胞的GSH依赖性抗氧化保护系统。
Adriamycin (ADR) failed to inhibit and paradoxically enhanced the biological action of 12-O-tetradecanoylphorbol-13-acetate (TPA) in mouse epidermis in vivo and in vitro. In the presence of ADR, the tumor promoter caused a greater sequential rapid increase and prolonged decrease in glutathione (GSH) peroxidase (GSH:H2O2oxidoreductase, EC 1.11.1.9) activity accompanied by a greater decrease in the ratio of reduced (GSH)/oxidized (GSSG) glutathione in isolated epidermal cells. The ability of ADR to deplete the intracellular level of GSH correlated with its ability to increase basal and TPA-induced ornithine decarboxylase (ODC,l-ornithine carboxylyase, EC 4.1.1.17) activities. In vivo, topical ADR treatments also enhanced TPA-induced ODC activity as well as the tumor-promoting ability of TPA in the two-stage system of mouse skin carcinogenesis. Since lipid peroxidation has been associated with ADR toxicity, these data suggest that the enhancement of the tumor-promoting ability of TPA by ADR may be the result of an increased oxidative challenge that overwhelms the GSH-dependent antioxidant protective system of the epidermal cells.
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影响因子：
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作者：
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影响因子：
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影响因子：
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作者：
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通讯作者：
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