Desiccation elicits heat shock protein transcription in the flesh fly, Sarcophaga crassipalpis, but does not enhance tolerance to high or low temperatures
Desiccation elicits heat shock protein transcription in the flesh fly, Sarcophaga crassipalpis, but does not enhance tolerance to high or low temperatures
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DOI：
10.1016/s0022-1910(99)00073-6
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发表时间：
1999-10-01
影响因子：
2.2
通讯作者：
Denlinger, DL
中科院分区：
文献类型：
--
作者：
Tammariello, SP;Rinehart, JP;Denlinger, DL
虽然热休克蛋白（hsp）的生产已被注意到在应对多种环境压力，没有链接之前已建立干燥和hsp之间。在0%相对湿度（RH）下非致命干燥后两种热休克蛋白转录本hsp23和hsp70在肉蝇Sarcophagacrassipis的蛹中上调达48 h。在对照组中几乎检测不到转录本，但在0%R.H.观察到较高转录物表达。这表明，对干燥的保护可能与肉蝇对其他环境应激源（如低温或高温）的一般应激反应有关。还测试了热休克蛋白诱导的干燥预处理后对冷休克和热休克的适应性交叉耐受性。虽然这两个热休克蛋白转录上调响应干燥，上调是不太戏剧性的上调引起的热休克，干燥未能产生耐受性高或低的温度。(C)1999爱思唯尔科技有限公司。保留所有权利。
Although heat shock protein (hsp) production has been noted in response to multiple environmental stresses, no Link has been previously established between desiccation and hsps. Following a nonlethal desiccation at 0% relative humidity (R.H.) for up to 48 h, two heat shock protein transcripts, hsp23 and hsp70, were upregulated in pupae of the flesh fly, Sarcophaga crassipalpis. The transcripts were nearly undetectable in control pupae, but within 24 h after being placed at 0% R.H. high transcript expression was observed. This suggests that protection against desiccation may be linked to the general stress response employed by flesh flies against other environmental stressors such as low or high temperature. Adaptive cross-tolerance to cold shock and heat shock following an hsp-inducing desiccation pretreatment was also tested. Although the two hsp transcripts were upregulated in response to desiccation, the upregulation was less dramatic than the upregulation elicited by heat shock, and desiccation failed to generate tolerance to high or low temperatures. (C) 1999 Elsevier Science Ltd. All rights reserved.