Sequence comparison of human and murine erythrocyte alpha-spectrin cDNA.
Sequence comparison of human and murine erythrocyte alpha-spectrin cDNA.
复制标题
人和鼠红细胞 α-血影蛋白 cDNA 的序列比较。
DOI：
10.1016/0378-1119(85)90191-x
复制
发表时间：
1985
期刊：
影响因子：
3.5
通讯作者：
Rovera,G
中科院分区：
文献类型：
--
作者：
Curtis,PJ;Palumbo,A;Ming,J;Fraser,P;Cioe,L;Meo,P;Shane,S;Rovera,G
用小鼠和人α-血影蛋白mRNA的cDNA探针进行杂交分析的结果表明，红细胞血影蛋白的α-亚基由一个基因编码。测序结果表明，它们编码370个氨基酸（aa），覆盖了α-血影蛋白C端附近的3个重复结构域。克隆的cDNA现在将允许分离α-血影蛋白基因，并将导致人类遗传性贫血的遗传方面的特征，其中α-血影蛋白已被表征为分子缺陷的位点。
The results of hybridization analyses using cDNA probes for mouse and human α-spectrin mRNA indicate that a single gene encodes the α-subunit of erythrocyte spectrin. Sequencing of the cDNA clones showed that they code for 370 amino acids (aa) covering three repeat domains close to the C terminus of α-spectrin. The cloned cDNAs will now permit the isolation of the α-spectrin gene and should lead to the characterization of the genetic aspects in human hereditary anemias in which α-spectrin has been characterized as the site of the molecular defect.