Onion yellow phytoplasma P38 protein plays a role in adhesion to the hosts.
Onion yellow phytoplasma P38 protein plays a role in adhesion to the hosts.
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DOI：
10.1111/1574-6968.12620
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发表时间：
2014-12
影响因子：
2.1
通讯作者：
Y. Neriya;K. Maejima;Takamichi Nijo;Tatsuya Tomomitsu;Akira Yusa;M. Himeno;O. Netsu;H. Hamamoto
中科院分区：
文献类型：
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作者：
Y. Neriya;K. Maejima;Takamichi Nijo;Tatsuya Tomomitsu;Akira Yusa;M. Himeno;O. Netsu;H. Hamamoto
粘附素是介导微生物病原体粘附到宿主细胞表面的微生物表面蛋白。在Mollicutes中，支原体和螺旋体中有几种粘连蛋白的报道。无乳支原体的粘附素P40和柑橘螺旋体的粘附素P89含有一个被称为Mollicutes adhesion motif （MAM）的保守氨基酸序列，其在宿主细胞粘附中的功能尚不清楚。在这里，我们发现植物原体，一种由昆虫媒介传播的植物致病性分子，具有含有粘附的MAM，该MAM在‘Candidatus Phytoplasma asteris ’ OY菌株的一个假定的膜蛋白PAM289 （P38）中被鉴定出来。P38同源物及其MAMs在相关植原体菌株中高度保守。P38蛋白在oy感染的昆虫和植物宿主中均有表达，结合实验表明P38蛋白与昆虫提取物相互作用，与植物提取物相互作用弱。有趣的是，P38与昆虫提取物的相互作用依赖于MAM。这些结果表明P38是一种与宿主相互作用的植原体粘附素。此外，粘附素的MAM对于P38蛋白与宿主的相互作用也很重要。
Adhesins are microbial surface proteins that mediate the adherence of microbial pathogens to host cell surfaces. In Mollicutes, several adhesins have been reported in mycoplasmas and spiroplasmas. Adhesins P40 of Mycoplasma agalactiae and P89 of Spiroplasma citri contain a conserved amino acid sequence known as the Mollicutes adhesin motif (MAM), whose function in the host cell adhesion remains unclear. Here, we show that phytoplasmas, which are plant-pathogenic mollicutes transmitted by insect vectors, possess an adhesion-containing MAM that was identified in a putative membrane protein, PAM289 (P38), of the 'Candidatus Phytoplasma asteris,' OY strain. P38 homologs and their MAMs were highly conserved in related phytoplasma strains. While P38 protein was expressed in OY-infected insect and plant hosts, binding assays showed that P38 interacts with insect extract, and weakly with plant extract. Interestingly, the interaction of P38 with the insect extract depended on MAM. These results suggest that P38 is a phytoplasma adhesin that interacts with the hosts. In addition, the MAM of adhesins is important for the interaction between P38 protein and hosts.