A regulatory protein that interferes with activator-stimulated transcription in bacteria
A regulatory protein that interferes with activator-stimulated transcription in bacteria
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DOI：
10.1073/pnas.0637648100
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发表时间：
2003-04-01
影响因子：
11.1
通讯作者：
Zuber, P
中科院分区：
文献类型：
--
作者：
Nakano, S;Nakano, MM;Zuber, P
细菌中的转录激活蛋白通常通过与RNA聚合酶α亚基（RNAP）的C-末端结构域相互作用而起作用。在这里，我们报告发现的“抗α”因子Spx在枯草芽孢杆菌中，通过结合到α-C-末端结构域，从而干扰RNAP的能力，以响应某些激活蛋白阻断转录激活。Spx破坏激活蛋白ResD和ComA与启动子结合的RNAP之间的复合物形成，并且它通过与α亚基的直接相互作用来实现。ResD和ComA刺激的转录需要通过ATP依赖性蛋白酶ClpXP蛋白水解消除Spx。Spx代表一类通过与α相互作用抑制激活子刺激的转录的转录调节因子。
Transcriptional activator proteins in bacteria often operate by interaction with the C-terminal domain of the alpha-subunit of RNA polymerase (RNAP). Here we report the discovery of an "anti-alpha" factor Spx in Bacillus subtilis that blocks transcriptional activation by binding to the alpha-C-terminal domain, thereby interfering with the capacity of RNAP to respond to certain activator proteins. Spx disrupts complex formation between the activator proteins ResD and ComA and promoter-bound RNAP, and it does so by direct interaction with the alpha-subunit. ResD- and ComA-stimulated transcription requires the proteolytic elimination of Spx by the ATP-dependent protease ClpXP. Spx represents a class of transcriptional regulators that inhibit activator-stimulated transcription by interaction with alpha.