The relationship between microvessel density, proliferative activity and expression of vascular endothelial growth factor-A and its receptors in eutopic endometrium and endometriotic lesions
The relationship between microvessel density, proliferative activity and expression of vascular endothelial growth factor-A and its receptors in eutopic endometrium and endometriotic lesions
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DOI：
10.1530/rep.1.01110
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发表时间：
2006-09-01
期刊：
影响因子：
3.8
通讯作者：
Ovsson, M.
中科院分区：
文献类型：
--
作者：
Bourley, V.;Volkov, N.;Ovsson, M.
本研究旨在探讨子宫内膜异位症和腹膜异位病变患者在位内膜微血管密度、增殖活性与血管生成的关系。并探讨血清和腹腔液中血管内皮生长因子-A(VEGF-A)水平是否反映了子宫内膜异位症病变组织中这些参数的变化。对25例子宫内膜异位症患者的在位内膜、腹膜异位内膜病变和14例非子宫内膜异位症患者的在位内膜组织标本进行免疫组织化学染色，观察间质、腺体和血管中血管内皮生长因子受体1和2(VEGFR-1和VEGFR-2)的表达及微血管密度、增殖活性的变化。测定腹腔液和血清中血管内皮生长因子-A的浓度。子宫内膜异位症患者分泌期在位内膜微血管密度、腺上皮中血管内皮生长因子受体-2的表达及血管内皮细胞生长因子受体-2的表达均显著高于非子宫内膜异位症患者。子宫内膜异位症高增殖活性病变与低增殖活性病变相比，微血管密度高，VEGFR-2血管表达高，腹腔液和血清中VEGF-A水平高。综上所述，子宫内膜异位症患者在位内膜可能存在血管生成活性异常，高增殖活性的异位内膜病变伴有局部血管生成活性增高，血清和腹腔液中的血管生成活性升高。
Studies were performed to elucidate the possible relationship between microvessel density, proliferative activity and angiogenesis in eutopic endometrium from women with and without endometriosis and peritoneal endometriotic lesions. The question whether changes in these parameters in endometriotic lesions were reflected by the level of vascular endothelial growth factor-A (VEGF-A) in serum and peritonea fluid was also studied. Biopsy specimens of both eutopic endometrium and peritoneal endometriotic lesions from women with endometriosis (n=25) as well as eutopic endometrium from women without endometriosis (n=14) were analysed immunohistochemically regarding microvessel density, proliferative activity, and expression of VEGF-A and its receptors vascular endothelial growth factor receptors 1 and 2 (VEGFR-1 and VEGFR-2) in stroma, glands and blood vessels. The VEGF-A concentration was measured in peritoneal fluid and serum. Secretory phase eutopic endometrium from women with endometriosis had significantly higher microvessel density, expression of VEGF-A in glandular epithelium and VEGFR-2 in endometrial blood vessels than those from women without endometriosis. Endometriotic lesions with high proliferative activity had a higher microvessel density and showed higher vascular expression of VEGFR-2 as well as being accompanied by higher levels of VEGF-A in peritoneal fluid and serum, compared with lesions with low proliferative activity. In conclusion, there seems to be a dysregulation of angiogenic activity in the eutopic endometrium of women with endometriosis and endometriotic lesions with high proliferative activity were accompanied by higher local angiogenic activity and higher levels of VEGF in serum and peritoneal fluid.