Sleep-waking discharge patterns of ventrolateral preoptic/anterior hypothalamic neurons in rats
Sleep-waking discharge patterns of ventrolateral preoptic/anterior hypothalamic neurons in rats
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DOI：
10.1016/s0006-8993(98)00631-3
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发表时间：
1998-08-24
期刊：
影响因子：
2.9
通讯作者：
McGinty, D
中科院分区：
文献类型：
--
作者：
Szymusiak, R;Alam, N;McGinty, D
实验动物的大量损伤、刺激和记录研究表明，视前区/下丘脑前部区（POA）内的神经元在调节睡眠诱导和睡眠维持中的重要性。最近，在大鼠的腹外侧POA （vlPOA）中发现一个离散的细胞簇在睡眠期间表现出升高的c-fos基因表达，表明这些神经元在非快速眼动和/或快速眼动睡眠阶段被强烈激活。我们使用慢性微丝技术检测了大鼠在清醒和睡眠时外侧POA背侧至腹侧范围内的神经元放电。我们发现，与清醒时相比，睡眠时放电率升高的神经元定位于vlPOA。作为一个整体，vlPOA神经元在非快速眼动和快速眼动睡眠期间都表现出较高的放电率。vlPOA神经元的放电反映了睡眠深度，即从浅睡眠到深非快速眼动睡眠，放电率显著增加。在12-14小时的睡眠剥夺后的恢复性睡眠中，与基线睡眠相比，vlPOA神经元显示出与睡眠相关的放电增加。睡眠时显示神经元放电增加的vlPOA神经元位于睡眠时c-fos基因表达增加的神经元所在的区域。这些神经元可能是调节睡眠开始和睡眠维持的吻侧下丘脑机制的组成部分。(C) 1998出版Elsevier Science B.V.版权所有
Numerous lesion, stimulation and recording studies in experimental animals demonstrate the importance of neurons within the preoptic/anterior hypothalamic area (POA) in the regulation of sleep induction and sleep maintenance. Recently, a discrete cluster of cells in the ventrolateral POA (vlPOA) of rats was found to exhibit elevated c-fos gene expression during sleep, indicating that these neurons are strongly activated during nonREM and/or REM sleep stages. We examined neuronal discharge during wakefulness and sleep throughout the dorsal to ventral extent of the lateral POA in rats, using chronic microwire technique. We found that neurons with elevated discharge rates during sleep, compared to waking, were localized to the vlPOA. As a group, vlPOA neurons displayed elevated discharge rates during both nonREM and REM sleep. Discharge of vlPOA neurons reflected the depth of sleep, i.e., discharge rates increased significantly from light to deep nonREM sleep. During recovery sleep following 12-14 h of sleep deprivation, vlPOA neurons displayed increased sleep-related discharge, compared to baseline sleep. Neurons in the vlPOA displaying increased neuronal discharge during sleep were located in the same area where neurons exhibit increased c-fos gene expression during sleep. Such neurons are likely components of a rostral hypothalamic mechanism that regulates sleep onset and sleep maintenance. (C) 1998 Published by Elsevier Science B.V. All rights reserved.