Long-term proliferation in culture and germline transmission of mouse male germline stem cells
Long-term proliferation in culture and germline transmission of mouse male germline stem cells
复制标题
DOI：
10.1095/biolreprod.103.017012
复制
发表时间：
2003-08-01
影响因子：
3.6
通讯作者：
Shinohara, T
中科院分区：
文献类型：
--
作者：
Kanatsu-Shinohara, M;Ogonuki, N;Shinohara, T
精子发生是一个复杂的过程，起源于一小群精原干细胞。在这里，我们报告了在体外培养的精原干细胞，增殖了很长一段时间。在胶质细胞源性神经营养因子、表皮生长因子、碱性成纤维细胞生长因子和白血病抑制因子存在的情况下，从新生小鼠睾丸分离的生殖细胞在5个月内增殖（>10（14）倍），并在移植到生精小管后恢复先天性不育受体小鼠的生育力。精原干细胞的长期培养将有助于研究精子发生机制，对转基因技术和医学新技术的发展具有重要意义。
Spermatogenesis is a complex process that originates in a small population of spermatogonial stem cells. Here we report the in vitro culture of spermatogonial stem cells that proliferate for long periods of time. In the presence of glial cell line-derived neurotrophic factor, epidermal growth factor, basic fibroblast growth factor, and leukemia inhibitory factor, gonocytes isolated from neonatal mouse testis proliferated over a 5-month period (>10(14)-fold)and restored fertility to congenitally infertile recipient mice following transplantation into seminiferous tubules. Long-term spermatogonial stem cell culture will be useful for studying spermatogenesis mechanism and has important implications for developing new technology in transgenesis or medicine.