Molecular phylogenetic identification of Fasciola flukes in Nepal
Molecular phylogenetic identification of Fasciola flukes in Nepal
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DOI：
10.1016/j.parint.2014.07.001
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发表时间：
2014-12-01
影响因子：
1.9
通讯作者：
Itagaki, Tadashi
中科院分区：
文献类型：
--
作者：
Shoriki, Takuya;Ichikawa-Seki, Madoka;Itagaki, Tadashi
对采自尼泊尔8个地区的81只片形吸虫的精子发生状态和核核糖体内转录间隔区1（ITS 1）进行了种间鉴定，并根据线粒体NADH脱氢酶亚基1（nad 1）基因分析了其与亚洲其他国家片形吸虫的系统发育关系。无精子片形吸虫61例（75.3%），20例（24.7%）为片形吸虫。所有的无精吸虫在ITS 1区均表现出Fh/Fg型，这在中国无精片形吸虫属中占优势;大多数（60个）吸虫在nad 1区表现出Fsp-ND 1-N1单倍型，其核苷酸序列与中国无精吸虫的主要单倍型（Fg-C2）完全相同。这些结果表明，无精子片形吸虫是从中国传入尼泊尔的。此外，多样性指数，中性指数和中位数加入网络分析的结果与参考单倍型从亚洲国家表明，无精子片形吸虫sp.迅速扩大其分布。相比之下，F.结果表明，中华绒螯蟹nad 1单倍型有10种，其种群多样性指数高于无精子吸虫单倍型，表明中华绒螯蟹nad 1单倍型的种群多样性指数高于无精子吸虫单倍型。在尼泊尔，无精子片形吸虫种群的分布明显早于无精子片形吸虫种群。虽然F.来自尼泊尔的Fg-ND 1-N1单倍型与Fg-ND 1-N1单倍型组成星型遗传。尼泊尔种群与缅甸和泰国的F.由于每个国家都有不同的创始人单倍型，因此邻近国家的南极洲种群。(C)2014爱思唯尔爱尔兰有限公司版权所有。
Eighty-one Fasciola flukes collected from 8 districts in Nepal were analyzed for their species identification on the basis of their spermatogenic status and nuclear ribosomal internal transcribed spacer 1 (ITS1) and for their phylogenetic relation with Fasciola flukes from other Asian countries on the basis of the mitochondrial NADH dehydrogenase subunit 1 (nad1) gene. Sixty-one flukes (75.3%) were aspermic Fasciola sp., and 20 flukes (24.7%) were identified as Fasciola gigantica. All of the aspermic flukes displayed the Fh/Fg type in ITS 1, which was predominant in aspermic Fasciola sp. from China, and most (60 flukes) displayed the Fsp-ND1-N1 haplotype in the nad1, which had an identical nucleotide sequence to the major haplotype (Fg-C2) of the aspermic flukes from China. These results suggest that aspermic Fasciola sp. was introduced into Nepal from China. Furthermore, the results of the diversity indices, neutrality indices, and median-joining network analysis with reference haplotypes from Asian countries suggest that aspermic Fasciola sp. rapidly expanded its distribution. In contrasts, F. gigantica displayed 10 nad1 haplotypes, which showed higher population diversity indices than the haplotypes of aspermic flukes, indicating that the F. gigantica population was clearly distributed in Nepal earlier than the aspermic Fasciola population. Although the F. gigantica haplotypes from Nepal formed a star-like phylogeny consisting of a main founder haplotype (Fg-ND1-N1), together with some F. gigantica haplotypes from Myanmar and Thailand, the Nepal population differed genetically from F. gigantica populations of neighboring countries as each country had distinct founder haplotype(s). (C) 2014 Elsevier Ireland Ltd. All rights reserved.