In vivo imaging of infection using a bacteria-targeting optical nanoprobe.
In vivo imaging of infection using a bacteria-targeting optical nanoprobe.
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DOI：
10.1166/jbn.2014.1852
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发表时间：
2014-05
影响因子：
2.9
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Tang EN;Nair A;Baker DW;Hu W;Zhou J
伤口和设备相关感染是发病和死亡的主要原因。因此，快速、早期诊断细菌定植对于感染治疗至关重要。然而，目前的诊断方法无法满足这一要求。因此，实际需要开发一种快速识别定植细菌的新方法。这项研究旨在开发能够实时检测和量化定植细菌数量的光学纳米探针。为此，我们合成了一种具有三个要素的成像纳米探针：作为细菌靶向配体的刀豆蛋白 A (Con A)、纳米颗粒载体和近红外荧光染料。 MTS 测定表明细菌纳米探针具有细胞相容性。体外测试进一步表明细菌纳米探针对细菌具有非常高的特异性和亲和力。使用小鼠伤口和导管感染模型，我们发现细菌纳米探针可以快速实时检测和量化伤口和导管上细菌定植的程度。
Wound and device-associated infection is a leading cause for morbidity and mortality. As such, rapid and early diagnosis of bacterial colonization is critical to infection treatment. The current diagnostic methods however, are not able to meet this requirement. Therefore, there is a practical need for the development of a new method to rapidly identify colonized bacteria. This study aims to develop optical nanoprobes that can detect and quantify the number of colonized bacteria in real time. To this end, we have synthesized an imaging nanoprobe with three elements: Concanavalin A (Con A) as a bacterial targeting ligand, a nanoparticle carrier, and a near infrared fluorescent dye. An MTS assay revealed that the bacteria nanoprobe is cell compatible. In vitro testing further showed that the bacteria nanoprobe had a very high specificity and affinity to bacteria. Using a murine wound and catheter infection model, we found that the bacteria nanoprobes can rapidly detect and quantify the extent of bacterial colonization on wounds and catheters in real time.
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