Types of Muscle Fibres in Toad Skeletal Muscle
Types of Muscle Fibres in Toad Skeletal Muscle
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蟾蜍骨骼肌的肌纤维类型
DOI：
10.1111/j.1748-1716.1966.tb03427.x
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发表时间：
1966
期刊：
影响因子：
--
通讯作者：
Richard S. Smith
中科院分区：
文献类型：
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作者：
J. Lännergren;Richard S. Smith
本文试图确定非洲爪蟾髂腓肠肌中存在的肌纤维类型。在肌肉横截面上进行组织化学研究。注意到细胞间脂质、dpn -脱氢酶和琥珀酸脱氢酶含量的差异，并以此为基础将纤维分为三大类。选择代表组织化学组的单个活纤维，研究它们在电刺激和乙酰胆碱应用下的收缩行为。研究结果表明了肌纤维的组织化学和功能特性之间的关系。脂质和酶含量适中的纤维（苍白纤维）是快速纤维，在持续刺激下迅速疲劳。脂质和酶含量高的纤维（暗纤维）是不容易疲劳的快纤维。不含脂质、不含dpn -脱氢酶或琥珀酸脱氢酶的纤维（透明纤维）为慢纤维。对纤维端板的染色表明，快纤维的端板大体形态各异，具有高胆碱酯酶活性，而慢纤维的端板呈“葡萄状”，胆碱酯酶活性非常低。
An attempt has been made to define the types of muscle fibres present in the iliofibularis muscle of Xenopus laevis. Histochemical studies were carried out on cross sections of the muscle. The variation between cells in the content of lipids, DPN-diaphorase and succinic dehydrogenase was noted, and on this basis the fibres were classified into three main groups. Single, living fibres representing the histochemical groups were selected and their contractile behaviour in response to electrical stimulation and the application of ACh was studied. The findings indicated a relationship between the histochemical and functional properties of the muscle fibres. Fibres with a moderate content of lipid and enzymes (pale fibres) were fast fibres which fatigued rapidly on maintained stimulation. Fibres with a high content of lipid and enzymes (dark fibres) were fast fibres which did not fatigue rapidly. Fibres containing no lipid and no DPN-diaphorase or succinic dehydrogenase (clear fibres) were slow fibres. Staining of the end-plates on the fibres showed that fast fibres had end-plates of varying gross morphology and with high cholinesterase activity, whereas slow fibres had ‘en grappe’ end-plates with very low cholinesterase activity.