NONRANDOM LOSS OF HUMAN MARKERS FROM MAN-MOUSE SOMATIC-CELL HYBRIDS
NONRANDOM LOSS OF HUMAN MARKERS FROM MAN-MOUSE SOMATIC-CELL HYBRIDS
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DOI：
10.1038/251072a0
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发表时间：
1974-01-01
期刊：
影响因子：
64.8
通讯作者：
MIGEON, BR
中科院分区：
文献类型：
--
作者：
NORUM, RA;MIGEON, BR
人类染色体在人和小鼠体细胞杂交1中优先丢失的现象被用于将人类基因位点分配到染色体2上。当实验设计不选择所研究的基因产物时，分配通常假设染色体的丢失是随机的。例如，由小鼠细胞和sv40转化的人类皮肤成纤维细胞衍生的杂交克隆只有在除少数人类染色体外的所有染色体都被清除后才会失去sv40诱导的T抗原。如果染色体丢失的模式是随机的，这些结果可能表明SV40 DNA已经整合到几条染色体的DNA中。然而，这可能是由于在每个克隆的进化过程中，一条相关的人类染色体在相对较晚的时间内被预定地消除，这一可能性得到了支持，因为在SV40转化的人类细胞衍生的杂交细胞中，C-7优先保留，并且这条染色体与SV40和T抗原4的存在一致。此外，如果染色体的丢失是直接的，可能会出现两条染色体的联合分离，这使得很难确定两条染色体中哪一条指定了相关基因产物同时丢失。
THE preferential loss of human chromosomes from hybrids between human and mouse somatic cells1is being used in the assignment of human gene loci to chromosomes2. When the experimental design does not select for the gene product under study, the assignment often assumes that the loss of chromosomes is random. For example, hybrid clones derived from mouse cells and SV40-transformed human skin fibroblasts lose the SV40-induced T antigen only when all but a few human chromosomes have been eliminated3. If the pattern of chromosome loss is random, these results could indicate that SV40 DNA has integrated into the DNA of several chromosomes. They might be attributable, however, to the scheduled elimination of a single pertinent human chromosome relatively late in the evolution of each clone, a possibility supported by the finding of a preferential retention of C-7 in hybrids derived from SV40-transformed human cells and a concordance of this chromosome with the presence of SV40 and T antigen4. Moreover, if the loss of chromosomes were directed, joint segregation of two chromosomes might occur, making it difficult to decide which of the two specified the pertinent gene product being lost simultaneously.