MOLECULAR-CLONING AND CHARACTERIZATION OF THE HUMAN BETA-LIKE GLOBIN GENE-CLUSTER
MOLECULAR-CLONING AND CHARACTERIZATION OF THE HUMAN BETA-LIKE GLOBIN GENE-CLUSTER
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DOI：
10.1016/0092-8674(80)90087-2
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发表时间：
1980-01-01
期刊：
影响因子：
64.5
通讯作者：
MANIATIS, T
中科院分区：
文献类型：
--
作者：
FRITSCH, EF;LAWN, RM;MANIATIS, T
编码人类胚胎（.epsilon.）、胎儿（G.gamma.）的基因。A.gamma)和成人（.delta., .beta.）度量。样珠蛋白多肽是从噬菌体中分离出来的一组重叠的克隆DNA片段。高分子量（15-20 kb）[千碱基]染色体DNA文库。在这些重叠的克隆中，65kb的DNA包含了所有5 . β的基因。类多肽，包括胚胎。-珠蛋白基因，其染色体位置以前是未知的。这5个基因都是从同一条DNA链转录而来，排列顺序为5”-.epsilon.-(13.3 kb)- g.g ama .-(3.5 kb)- a.g ama .-(13.9 kb)-.delta.-(5.4 kb)-.beta.-3”。因此，这些基因在发育过程中按其表达顺序排列在染色体上。除了5个已知的。比如珠蛋白基因，另外2个。样珠蛋白序列检测不对应于已知的多肽。其中一个序列被映射到a。γ。- δ。另一个位于6-9 KB - 5”的基因间区。基因。基因簇的基因间杂交实验显示一个非红蛋白重复序列（*），该序列以以下方式与珠蛋白基因穿插：5“-**.epsilon.-* g.g ama .- a.g ama .*-**.delta.-.beta.*-3”。对位于。delta.-globin基因5英寸的区域进行精细结构定位，发现2个重复序列，最大长度为400 bp[碱基对]，由。apprx隔开。700bp的DNA在集群内没有重复。初步实验表明，该重复家族在人类基因组中也重复多次。
The genes encoding human embryonic (.epsilon.), fetal (G.gamma., A.gamma.) and adult (.delta., .beta.) .beta.-like globin polypeptides were isolated as a set of overlapping cloned DNA fragments from bacteriophage .lambda. libraries of high MW (15-20 kb) [kilobases] chromosomal DNA. The 65 kb of DNA represented in these overlapping clones contains the genes for all 5 .beta.-like polypeptides, including the embryonic .epsilon.-globin gene, for which the chromosomal location was previously unknown. All 5 genes are transcribed from the same DNA strand and are arranged in the order 5''-.epsilon.-(13.3 kb)-G.gamma.-(3.5 kb)-A.gamma.-(13.9 kb)-.delta.-(5.4 kb)-.beta.-3''. Thus, the genes are positioned on the chromosome in the order of their expression during development. In addition to the 5 known .beta.-like globin genes, 2 other .beta.-like globin sequences were detected which do not correspond to known polypeptides. One of these sequences was mapped to the A.gamma.-.delta. intergenic region while the other is located 6-9 kb 5'' to the .epsilon. gene. Cross hybridization experiments between the intergenic sequences of the gene cluster revealed a nonglobin repeat sequence (*) which is interspersed with the globin genes in the following manner: 5''-**.epsilon.-*G.gamma.-A.gamma.*-**.delta.-.beta.*-3''. Fine structure mapping of the region located 5'' to the .delta.-globin gene revealed 2 repeats with a maximum size of 400 bp [base pairs] which are separated by .apprx. 700 bp of DNA not repeated within the cluster. Preliminary experiments indicate that this repeat family is also repeated many times in the human genome.