Detection of Borrelia burgdorferi, Anaplasma phagocytophilum and Babesia microti, with two different multiplex PCR assays
Detection of Borrelia burgdorferi, Anaplasma phagocytophilum and Babesia microti, with two different multiplex PCR assays
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DOI：
10.1016/j.ttbdis.2013.12.001
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发表时间：
2014-01-01
影响因子：
3.2
通讯作者：
Piesman, Joseph
中科院分区：
文献类型：
--
作者：
Hojgaard, Andrias;Lukacik, Gary;Piesman, Joseph
我们建立了检测伯氏疏螺旋体、吞噬无形体和微小巴贝斯虫的两种实时多重聚合酶链式反应方法。使用现场采集的对每种病原体呈阳性的肩部硬蜱、携带每一种聚合酶链式反应靶标的克隆质粒以及感染伯氏杆菌B31的实验室肩部硬蜱对每一种多重PCR方法的效率和灵敏度进行评估。多重聚合酶链式反应与单项聚合酶链式反应在效率和灵敏度上无明显差异。如果在同一分析中使用这两种多重聚合酶链式反应方法，现场采集的只携带宫本氏杆菌的扁虱也可以被识别出来。与单靶点聚合酶链式反应相比，多重检测方法是一种快速、经济有效的方法，用于筛查和检测硬蜱中的病原体。由Elsevier GmbH出版。
We have developed 2 real-time multiplex PCR assays for detection of Borrelia burgdorferi, Anaplasma phagocytophilum, and Babesia microti. The efficiency and sensitivity of each multiplex PCR assay was evaluated using field-collected Ixodes scapularis ticks that were positive for each of the pathogens, cloned plasmids harboring each of the PCR targets, and laboratory I. scapularis infected with B. burgdorferi B31. There was no difference in efficiency or sensitivity when comparing the multiplex PCR with the individual PCR reactions. If the 2 multiplex PCR assays are used in the same analysis, field-collected ticks that only harbor B. miyamotoi can also be identified. The multiplex assays are fast and cost-effective methods for screening and detecting pathogens in ticks, when compared to single-target PCR. Published by Elsevier GmbH.