Molecular characterization of CDC42, a Saccharomyces cerevisiae gene involved in the development of cell polarity.
Molecular characterization of CDC42, a Saccharomyces cerevisiae gene involved in the development of cell polarity.
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DOI：
10.1083/jcb.111.1.143
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发表时间：
1990-07
影响因子：
7.8
通讯作者：
Pringle, J R
中科院分区：
文献类型：
--
作者：
Johnson, D I;Pringle, J R
酿酒酵母CDC 42基因产物参与细胞分裂周期的形态发生事件;温度敏感性CDC 42突变体在限制性温度下不能形成芽并显示离域细胞表面沉积（亚当斯，A. E. M.，D. I.约翰逊河M.朗内克，B。F. Sloat和J.R.普林格尔1990. 111：131-142）。为了开始对CDC 42功能的分子分析，我们从酵母基因组DNA文库中分离了CDC 42基因。使用克隆的DNA来产生CDC 42的缺失，证实了该基因是必需的。GAL 10启动子控制下的CDC 42的过表达对细胞生长没有严重的危害，但确实扰乱了出芽位点选择的正常模式。CDC 42的DNA和预测的氨基酸序列的测定揭示了氨基酸序列与ras和rho的高度相似性（Madaule，P.，R.阿克塞尔和A. M.迈尔斯1987. Proc. Natl. Acad. Sci. 84：779-783）基因产物家族。ras蛋白的相似性（大约40%相同或相关的氨基酸）是最明显的区域，涉及GTP结合和水解，并在COOH-末端修饰导致膜协会，这表明CDC 42功能也涉及这些生化特性。与rho蛋白的相似性（总体上约60%相同或相关的氨基酸）更广泛地分布在编码区，这表明在尚未确定的生化特性和功能方面存在更广泛的相似性。
The Saccharomyces cerevisiae CDC42 gene product is involved in the morphogenetic events of the cell division cycle; temperature-sensitive cdc42 mutants are unable to form buds and display delocalized cell- surface deposition at the restrictive temperature (Adams, A. E. M., D. I. Johnson, R. M. Longnecker, B. F. Sloat, and J. R. Pringle. 1990. J. Cell Biol. 111:131-142). To begin a molecular analysis of CDC42 function, we have isolated the CDC42 gene from a yeast genomic DNA library. The use of the cloned DNA to create a deletion of CDC42 confirmed that the gene is essential. Overexpression of CDC42 under control of the GAL10 promoter was not grossly deleterious to cell growth but did perturb the normal pattern of selection of budding sites. Determination of the DNA and predicted amino acid sequences of CDC42 revealed a high degree of similarity in amino acid sequence to the ras and rho (Madaule, P., R. Axel, and A. M. Myers. 1987. Proc. Natl. Acad. Sci. 84:779-783) families of gene products. The similarities to ras proteins (approximately 40% identical or related amino acids overall) were most pronounced in the regions that have been implicated in GTP binding and hydrolysis and in the COOH-terminal modifications leading to membrane association, suggesting that CDC42 function also involves these biochemical properties. The similarities to the rho proteins (approximately 60% identical or related amino acids overall) were more widely distributed through the coding region, suggesting more extensive similarities in as yet undefined biochemical properties and functions.