A system for studying genetic changes in Candida albicans during infection
A system for studying genetic changes in Candida albicans during infection
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DOI：
10.1016/s1087-1845(02)00585-6
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发表时间：
2003-06-01
影响因子：
3
通讯作者：
Magee, PT
中科院分区：
文献类型：
--
作者：
Forche, A;May, G;Magee, PT
白念珠菌是一种具有二态生活史的二倍体酵母。它普遍存在于许多健康的人类中，但在免疫受损的宿主中成为一种强有力的病原体。白念珠菌在宿主中从共生状态转换为致病状态的潜在遗传机制尚不清楚。为了研究哺乳动物宿主毒力的进化，我们使用GAL1作为选择标记系统，允许在选择介质中进行正选择和负选择。我们发现，白念珠菌基因组中GAL1的一个或两个副本的缺失不会改变系统性小鼠模型的毒力。我们获得了系统感染期间GAL1基因有丝分裂重组频率的估计值。我们的观察表明，有丝分裂重组和基因转换等遗传变化发生的频率足够高，在白念珠菌从共生体向致病生物体的转变过程中非常重要。(C)2002年埃尔塞维尔科学公司(美国)。版权所有。
Candida albicans is a diploid yeast with a dimorphic life history. It exists commensally in many healthy humans but becomes a potent pathogen in immunocompromised hosts. The underlying genetic mechanisms by which C albicans switches from a commensal to a pathogenic form in the host are not well understood. To study the evolution of virulence in mammalian hosts, we used GAL1 as selectable marker system that allows for both positive and negative selection in selective media. We show that the deletion of one or both copies of GAL1 in the C albicans genome does not change virulence in a systemic mouse model. We obtained estimates for the frequency of mitotic recombination at the GAL1 locus during systemic infection. Our observations suggest that genetic changes such as mitotic recombination and gene conversion occur at a high enough frequency to be important in the transition of C albicans from a commensal to a pathogenic organism. (C) 2002 Elsevier Science (USA). All rights reserved.