Characterization of Romboutsia ilealis gen. nov., sp nov., isolated from the gastro-intestinal tract of a rat, and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov and Asaccharospora gen. nov.
Characterization of Romboutsia ilealis gen. nov., sp nov., isolated from the gastro-intestinal tract of a rat, and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov and Asaccharospora gen. nov.
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DOI：
10.1099/ijs.0.059543-0
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发表时间：
2014-05-01
影响因子：
2.8
通讯作者：
Smidt, Hauke
中科院分区：
文献类型：
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作者：
Gerritsen, Jacoline;Fuentes, Susana;Smidt, Hauke
从大鼠胃肠道中分离出一种革兰氏阳性染色、杆状、非运动、孢子形成的专性厌氧细菌，命名为CRIBT，并进行了表征。CRIBT菌株的主要细胞脂肪酸是饱和和不饱和的直链C-12-C-19脂肪酸，其中C-16：0是主要的脂肪酸。极性脂质谱包含6种糖脂、4种磷脂和1种不被所用任何特定喷雾试剂染色的脂质。唯一的醌是MK-6。主要的细胞壁糖是葡萄糖和半乳糖。CRIBT菌株的肽聚糖类型为A1 sigma羊毛硫氨酸直接型。CRIBT基因组DNA G+C含量为28.1mol%。基于16 S rRNA基因序列相似性，菌株CRIBT与梭菌属的许多种最密切相关，包括Clostridium lituseburense（97.2%）、Clostridium glycolicum（96.2%）、Clostridium mayombei（96.2%）、Clostridium bartlettii（96.0%）和Clostridium irregulare（95.5%）。所有这些物种显示非常低的16 S rRNA基因序列相似性（
A Gram-positive staining, rod-shaped, non-motile, spore-forming obligately anaerobic bacterium, designated CRIBT, was isolated from the gastro-intestinal tract of a rat and characterized. The major cellular fatty acids of strain CRIBT were saturated and unsaturated straight-chain C-12-C-19 fatty acids, with C-16:0 being the predominant fatty acid. The polar lipid profile comprised six glycolipids, four phospholipids and one lipid that did not stain with any of the specific spray reagents used. The only quinone was MK-6. The predominating cell-wall sugars were glucose and galactose. The peptidoglycan type of strain CRIBT was A1 sigma lanthionine-direct. The genonnic DNA G+C content of strain CRIBT was 28.1 mol%. On the basis of 16S rRNA gene sequence similarity, strain CRIBT was most closely related to a number of species of the genus Clostridium, including Clostridium lituseburense (97.2 %), Clostridium glycolicum (96.2%), Clostridium mayombei (96.2 %), Clostridium bartlettii (96.0 %) and Clostridium irregulare (95.5 %). All these species show very low 16S rRNA gene sequence similarity (