Suppression of in vivo polyclonal IgE responses by monoclonal antibody to the lymphokine B-cell stimulatory factor 1.
Suppression of in vivo polyclonal IgE responses by monoclonal antibody to the lymphokine B-cell stimulatory factor 1.
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通过针对淋巴因子 B 细胞刺激因子 1 的单克隆抗体抑制体内多克隆 IgE 反应。
DOI：
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发表时间：
1986
影响因子：
11.1
通讯作者：
W. Paul
中科院分区：
文献类型：
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作者：
F. Finkelman;I. Katona;J. Urban;C. Snapper;J. Ohara;W. Paul
淋巴因子B细胞刺激因子1(BSF-1)在体外能显著促进脂多糖激活的B细胞分化为IgG1和IgE分泌细胞。为了确定体内的IgG1和IgE抗体反应是否依赖于BSF-1，研究了大鼠抗BSF-1的单抗11B11对感染巴西拟青霉线虫的小鼠多克隆IgG1和IgE产生的影响以及对小鼠免疫球蛋白D纯化的山羊抗体的影响。含11B11的腹水和纯化的11B11对两个系统的IgE生成均有强烈的抑制作用，但不影响IgG1的生成，而对照组腹水和正常大鼠的IgG1则不具有抑制IgE的活性。这些结果表明BSF-1在产生IgE抗体反应中发挥了重要的生理作用，并建议了在不抑制保护性抗体反应的情况下限制引起过敏反应的抗体的产生的方法。
The lymphokine B-cell stimulatory factor 1 (BSF-1) has been shown to greatly enhance the differentiation of lipopolysaccharide-activated B cells into IgG1- and IgE-secreting cells in vitro. To determine whether in vivo IgG1 and IgE antibody responses are BSF-1 dependent, the ability of a monoclonal rat IgG1 anti-BSF-1 antibody, 11B11, to affect polyclonal IgG1 and IgE production in mice infected with the nematode parasite Nippostrongylus brasiliensis or injected with a purified goat antibody to mouse IgD was studied. 11B11-containing ascites fluid or purified 11B11 strongly inhibited IgE production in both systems but did not affect IgG1 production, while control ascites or normal rat IgG1 had no IgE-inhibitory activity. These results indicate an important physiologic role for BSF-1 in the generation of IgE antibody responses and suggest means for limiting the production of antibodies responsible for allergic reactions without inhibiting protective antibody responses.